نتایج جستجو برای: recombinant expression
تعداد نتایج: 951158 فیلتر نتایج به سال:
Abstract Background and Objective: Hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. Recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. The aim of this study was to evaluate the effect of inserting protein A signal peptide sequence of pEZZ18 plasmid on expression and secretion...
an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...
Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants. Objective: The main goal of the present study was to eva...
Background and purpose: Rapid emergence of traditional antibiotic-resistant pathogens is one of the most important global challenges in medical sciences. To this end, substitution of current antibiotics with strong antimicrobial peptides could be of great benefit. Materials and methods: In this study, the DNA sequence encoding dermaseptin B1 (DrsB1) antimicrobial peptide derived from Phyllomed...
Background and Objective: In recent years, the biological methods have been considered as a most promising candidate for the cancer treatment. One of the most applicable methods is recombinant proteins. ONTAK immunotoxin is a special drug for cancer therapy of leukemia, lymphoma and melanoma. With respect to the dramatic growth of cancer in today's society, production of recombinant prot...
Background: Human luteinizing hormone (hLH) stimulates steroid biosynthesis of the ovary, triggers ovulation and prepares androgen production of testicular Leydig cells. LH belongs to the family of glycoprotein hormones that are heterodimers consisting of a common α-subunit and specific β-subunit. hLH is necessary for clinical and infertility treatment. Recombinant DNA technology provides a use...
the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...
Introduction: Hepatitis C virus (HCV) infection is a major public health problem which influences about 170 million people worldwide. Different types of vaccines have been designed using HCV structural proteins to control the viral infection. The core nucleocapsid protein is one of the most conserved proteins and a desirable target for HCV vaccines, especially the protein-based vaccines. In cur...
Recently, new molecular breeding and genetic engineering approaches have emerged to overcome the limitations of conventional breeding methods in generating disease-resistance transgenic plants. The use of antimicrobial peptides (AMPs) to produce transgenic plants resistant to a wide range of plant pathogens has achieved great success. Among huge number of AMPs, Dermaseptin B1 (DrsB1), an antimi...
hepatitis c virus (hcv) is an important agent causing chronic liver infection, which often leads to liver cirrhosis and lethal hepatocellular carcinoma (hcc). at present, there is no effective hcv vaccine for prevention of hepatic disease and the standard treatment is neither economical nor fully effective in all the patients. however, vaccination based on structural and nonstructural proteins ...
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