Biomolecular interactions may be defined at several levels. The simplest quantitative descriptor, affinity, is defined by the affinity constant, KD, a measure of the strength of binding at equilibrium. This term may be further resolved into kinetic descriptors of rate of association (defined by the association rate constant (ka) and dissociation (kd). Kinetic information, however, does not reve...

The abilities of organisms to sequester substrate are described by the two kinetic constants specific affinity, a degrees, and maximal velocity Vmax. Specific affinity is derived from the frequency of substrate-molecule collisions with permease sites on the cell surface at subsaturating concentrations of substrates. Vmax is derived from the number of permeases and the effective residence time, ...

     Most of the biological phenomena are influenced by intermolecular recognition and interaction. Thus, understanding the thermodynamics of biomacromolecule ligand interaction is a very interesting area in biochemistry and biotechnology. One of the most powerful techniques to obtain precise information about the energetics of (bio) molecules binding to other biological macromolecules is isoth...

This paper indicates that activated sludge under certain conditions can achieve levels of polyhydroxyalkanoates (PHA) accumulation which would enable the economic recovery of this precursor of biodegradable plastics. The effects of C/N ratio and temperature on polyhydroxyalkanoates production were evaluated. Acetate uptake, ammonium consumption, oxygen uptake and PHA production were studied und...

AbstractInteraction of Ni complex(Salen= N, N´-ethylene bis(salicylideneimine)) with hen egg-white lysozyme (HEWL) was studied by absorption spectroscopy, competitive binding study and thermal denaturation study. The protein binding affinity of Ni complex was found to be (3.0×103M−1). The binding plot obtained from the absorption titration data gives a binding constant of 2.4 (± 0.3)×103 M...

The N-(2-benzoyl-phenyl) oxalyl derivatives are important models for studying of three-centered intramolecular hydrogen bonding in organic molecules. The quantum theoretical calculations for two crystal structures of N-(2-benzoyl-phenyl) oxalyl (compounds I and II) were performed by Density Functional Theory (B3LYP method and 6-311+G* basis set). From the optimized structures, geometric paramet...

in this study we produced and purified a high titer of specific and high affin¬ity fab fragments of anti-digoxin antibody. immunization of rabbits with a conju¬gate of the cardiac glycoside digoxin, coupled by a periodate oxidation method to the amino group of lysine in bovine serum albumin resulted in the production of this type of high titer digoxin-specific antibodies with exceptionally high...