In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...

The ultradian rhythm of growth hormone (GH) secretion has been known in several animal species for years and has recently been observed in cattle. Although the physiological significance of the rhythm is not yet fully understood, it appears essential for normal growth. In this review, previous studies concerning the GH secretory pattern in cattle, including its ultradian rhythm, are introduced ...

there are numerous, often contradictory reports on the effect of growth hormone (gh) therapy on thyroid function. these reports prompted us to evaluate the impact of gh therapy on thyroid function in previously euthyroid children with gh deficiency. twenty five clinically and biochemically euthyroid children with gh deficiency were studied. a thyroid profile (t4, free t4, t3 and tsh) was perfor...

introduction: fasting during the holy month of ramadan is an obligation for all adult, healthy muslims. as several studies have indicated, both fasting and energy restriction can alter thyroid hormone metabolism and affect the clinical features and well-being of hypothyroid patients. hypothyroidism is more prevalent among women and the elderly. its prevalence has been reported to be 4-8% of the...

We previously reported the chronological appearance of adenohypophysial cells in several vertebrates using an immunocytochemical technique. The present study investigated the chronological appearance of adenohypophysial cells in the freshwater Japanese soft-shelled turtle (Pelodiscus sinensis japonicus). The results were compared with those of the sea turtle, as well as other vertebrates which ...

background: in the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. signal peptides play a critical role in prosperous secretion of recombinant proteins. accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-er...

This study investigated the effects of five levels of galanin on plasma concentrations of growth hormone, triiodothyronine, thyroxine, and milk protein in dairy goat breed was sannan. 15 breeds of dairy goats sannan all about the same age and weight were selected for this study and were randomly divided into five treatments (three replicates per treatment). Levels of the hormone injection of ga...