Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR reactions. From this array the number of reactions that contain at least one (as opposed to zero) initial template is determined, allowing inferring the original template concentration. Here we present a novel protocol to efficiently infer the concentration of a sample and its optimal dilution for dPCR from few tar...

The replacement of steam catapults with electromagnetic ones is becoming an overwhelming trend in aircraft launch systems. The Electromagnetic Aircraft Launch System (EMALS) offers significant benefits to the aircraft, ship, personnel, and operational capabilities. EMALS has such advantages as high thrust, good controllability, reusable, etc., as a launching motor, a double-side plate Permanent...

Recent literature has reported that high concentrations of indicator bacteria such as fecal coliforms (FCs) were measured in anaerobically digested sludges immediately after dewatering even though low concentrations were measured prior to dewatering. This research hypothesized that the indicator bacteria can enter a non-culturable state during digestion, and are reactivated during centrifuge de...

As 5G millimeter wave (mmWave) wireless device involves some new technologies, such as beamforming, the radiofrequency (RF) exposure compliance test for mmWave is significantly complicated. In order to shorten period terminals, relevant regulatory authorities recommend a combination of numerical simulation and measurements demonstrate compliance. To verify feasibility this method, RF conducted ...

The present study describes the changes in expression of hydroxy- carboxylic acid receptor 2 (HCA2 receptor) in femoral epiphysis and metaphysis of rats with glucocorticoid-induced osteoporosis (GIO). 16 growing male Sprauge dawley rats were randomly divided into two equal groups consisting of normal control and rats that were rendered osteoporotic by receiving 0.1 mg/kg/day dexamethasone throu...

the taguchi method was applied as an experimental design to determine optimum conditions for colour removal from azo dyes solutions containing remazol yellow g by electrocoagulation (ec) using iron and steel electrodes as anode and cathode, respectively. an orthogonal array (oa9) experimental design that allows to investigation the simultaneous variations of four parameters (initial remazol ye...