Estimation of genetic trends is necessary to monitor and evaluate selection programs. The objective of this study was to estimate the genetic trends for milk yield in Iranian Holsteins cows using random regression test day model. Data set was consisted of 743205 test-day records from 1991 to 2008, which were collected by the Animal Breeding Centre of Iran. Breeding, environmental and phenotypic...

An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304'AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification a...

Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking ...

Plant transformation technology has become a versatile platform for cultivar improvement as well as for studying gene function in plants. The development of an efficient method for genetic transformation is a prerequisite for the application of molecular biology to the improvement of a given crop species. Agrobacterium-mediated genetic transformation is the dominant technology used for the prod...

Ethylene evolution from plants inhibits Agrobacterium-mediated genetic transformation, but the mechanism is little understood. In this study, the possible role of ethylene in Agrobacterium-mediated genetic transformation was clarified. It was tested whether or not plant ethylene sensitivity affected genetic transformation; the sensitivity might regulate bacterial growth during co-cultivation an...

The difficulty to dissect a complex phenotype of established malignant cells to several critical transcriptional programs greatly impedes our understanding of the malignant transformation. The genetic elements required to transform some primary human cells to a tumorigenic state were described in several recent studies. We took the advantage of the global genomic profiling approach and tried to...

the objective of this research was to estimate the genetic parameters and trend of somatic cell count in holstein cows of iran using test day records. the phenotypic the used data consisted of were consisting of 407167 first lactation test day records the which were collected by the center of animal breeding and livestock production improvement center of iran during 2009 to 2013. somatic cell c...