In this study, we focused on an inactivation process of HeLa cells by generated chemical species in a culture medium using a plasma flow. The plasma flow was formed between a platinum needle electrode 0.3 mm in diameter and the surface of the culture medium with 7.5 kV of positive voltage for 210 seconds. When the cells were incubated continuously in the treated culture medium, all of them were...

The horse-adapted virulent Bucyrus (VB) strain of equine arteritis virus (EAV) established persistent infection in high-passage-number human cervix cells (HeLa-H cells; passages 170 to 221) but not in low-passage-number human cervix cells (HeLa-L cells; passages 95 to 115) or in several other cell lines that were evaluated. However, virus recovered from the 80th passage of the persistently infe...

Development of a biomimetic 3D culture system for drug screening is necessary to fully understand the in vivo environment. Previously, a self-assembling peptide hydrogel has been reported; the hydrogel exhibited physiological properties superior to a 3D cell culture matrix. In this work, further research using H9e hydrogel with HeLa cells was carried out considering H9e hydrogel's interaction w...

Parameters that affect production of the recombinant reporter protein, EGFP, in the T7 promoter based VOTE vaccinia virus-HeLa cell expression system were examined. Length of infection phase, inducer concentration, and timing of its addition relative to infection were evaluated in 6-well plate monolayer cultures. One hour infection with 1.0 mM IPTG added at the time of infection provided a robu...

background: cross-contamination between cells is a usual mistake at cell culture laboratories and cell banks worldwide. mrc5 diploid cell and rk-13, vero and hela continuous cell lines are used in different stages of human viral vaccines propagation at razi vaccine and serum research institute of iran. however diploid and continuous cells are propagated at separated cell culture laboratory and ...

Salvia chorassanica Bunge is one of the Iranian endemic species of Salvia. There is not any reported literature on S. chorassanica. This study was designed to examine the in-vitro anti-proliferative and proapoptotic effects of the methanol extract of S. chorassanica and its fractions on HeLa cell line. Cells were cultured in EX-CELL®, an animal free medium specially designed for HeLa cell line ...

There is an ever increasing need to find surfaces that are biocompatible for applications like medical implants and microfluidics-based cell culture systems. The biocompatibility of five different surfaces with different hydrophobicity was determined using gene expression profiling as well as more conventional methods to determine biocompatibility such as cellular growth rate, morphology and th...

grown in tissue culture has been amply demon strated by the numerous reports in the literature on the HeLa strain of epithelial cell developed by Gey and co-workers (.5). This report will describe the culture characteristics of three more human tissue culture cell strains derived from epidermoid cancers, one embryonal nhabdomyosareoma , and one sarcoma which, as yet, has not been com pletely ad...

The IG20 gene encodes at least four splice variants, including DENN-SV and IG20. DENN-SV is constitutively expressed at higher levels in tumor tissues. Cells transfected with the DENN-SV cDNA show increased resistance to tumor necrosis factor (TNF ), TNF-related apoptosis-inducing ligand (TRAIL), etoposide, and vinblastine treatment, whereas overexpression of IG20 enhanced susceptibility to bot...