Enterohemorragic Escherichia coli strains (EHEC) represent an important problem for public health in developed countries. Indeed, EHEC strains can infect humans via vegetal and animal food consumption and cause food poisoning with diarrhoeas, frequently accompanied by hemorrhagic colitis with, in 10 % of the cases, apparition of renal sequelae (Haemolytic Uraemic Syndrome) that can lead to deat...

Adherence of enterohemorrhagic Escherichia coli (EHEC) to intestinal epithelium is essential for initiation of the infection. To identify genes involved in adherence, an EHEC O157:H7 strain (O157Sakai) was mutagenized by mini-Tn5Km2, where Km refers to kanamycin resistance, and 4,677 insertion mutants were screened for their ability to form microcolonies (MC) on Caco-2 cells. The less adherent ...

Diarrheal diseases still remain health problem worldwide and out of many bacteria responsible for, Shigella and pathogenic Escherichia cause the most diarrheas in the world. Shigellacause bacterial dysenteries and shigellosis through invasion where the most effective proteins for pathogenesis is Ipac. Critical virulence protein for ETEC infection is CFA/I with two subunits called cfab and cfae....