background: platelet mps are produced automatically during platelet storage. their size is about 0.1 – 1 µm and have ability for sub- endothelium adhering, strengthen of platelet aggregation and also platelet pre- coagulation. in fact, the release of membrane particles helps to signal to the neighbor cells or to seclude target cells from apoptosis. the purpose of this study is to determine the ...

background: routine repeat testing of critical laboratory values is very common these days to increase their accuracy and to avoid reporting false or infeasible results. we figure that repeat testing of critical laboratory values has any benefits or not.   methods : we examined 2233 repeated critical laboratory values in 13 different hematology and chemistry tests including: hemoglobin, white b...

A quantitative immunofluorescence platelet-associated immunoglobulins (PA-IgG) assay was used to detect alloimmunity to platelets in 8/12 multitransfused patients and to perform platelet crossmatching in the 8 alloimmunized patients. The correct separation of multitransfused patients into alloimmune and nonalloimmune groups was substantiated with chromium-51-labeled platelet survival studies. F...

A monoclonal antibody directed against the von Willebrand factor moiety (vWF) of factor VIII-von Willebrand factor (FVIII-vWF), which blocks ristocetin-induced platelet aggregation as well as the binding of FVIII-vWF to platelets in the presence of ristocetin, inhibited platelet adherence to human artery subendothelium when present in normal flowing blood. This monoclonal antibody, CLB-RAg 35, ...

To investigate the possibility that thrombin and/or other platelet activators change the platelet surface expression of glycoprotein IV (GPIV, CD36), we used a panel of five GPIV-specific monoclonal antibodies (OKM5, 5F1, FA6-152, 8A6, and F13) directed against different epitopes. All these antibodies bound to resting platelets in a concentration-dependent and saturable manner, as determined by...

Plugging of the capillary bed can lead to organ failure and mortality in sepsis. We have reported that intravenous ascorbate injection reduces platelet adhesion to the capillary wall and capillary plugging in septic mice. Both platelet adhesion and capillary plugging require P-selectin, a key adhesion molecule. To elucidate the beneficial effect of ascorbate, we hypothesized that ascorbate redu...