BACKGROUND With the appreciation of the high prevalence of coeliac disease there is increasing use of serology in screening asymptomatic people and testing those with suggestive features. AIM To compare the sensitivities and specificities of the endomysial antibody and the tissue transglutaminase antibody tests. METHODS Using electronic databases a search was made for relevant papers using ...

omalizumab is a recombinant humanized anti-ig e monoclonal antibody used as the third line treatment of chronic spontaneous urticaria (csu). we report four patients with severe antihistamine-resistant csu, who developed angioedema, anaphylaxis and/or flare up of urticaria at different times following omalizumab therapy.

background and aims: since the produced recombinant proteins by molecular genetics techniques commonly have some limitations in the application, chimeric protein are introduced. chimeric proteins have found widespread application for the study of protein folding, structure stability, function and immunogenisity. methods: according to the known immunomodulatory effect and structure of hsp70 mole...

epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a  chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...

background: erythropoietin (epo) was first known as a factor for red blood cell proliferation and differentiation. new studies show the effects of epo on immune system. objective: in this study, the effects of pretreatment with recombinant human erythropoietin (rhuepo) on the anti-human leukocyte antibody (anti-hla) titer were determined. methods: three groups of rats were sensitized with human...

Several diagnostic methods including immunofluorescence, enzyme-linked immunosorbent assay, polymerase chain reaction and immunohistochemistry have been developed for the detection of porcine epidemic diarrhea virus (PEDV). An immunohistochemical method using a new recombinant antibody produced by a phage antibody system (PAS16) kit was investigated and compared with that using a monoclonal ant...

Visceral leishmaniasis (VL) is a zoonotic disease caused by leishmania species. Dogs are considered to be the main reservoir of VL. A number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. However, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. A variety of serological tests ...