We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites wit...

The rapid evolution of bacteria is crucial to their survival and is caused by exchange, transfer, and uptake of DNA, among other things. Conjugation is one of the main mechanisms by which bacteria share their DNA, and it is thought to be controlled by varied bacterial immune systems. Contradictory results about restriction-modification systems based on phenotypic studies have been presented as ...

this experiment was conducted to compare the effects of quantitative feed restriction and meal feeding on the performance, carcass compositions and ascites syndrome in male broiler chickens (ross 308). in this study, 5 schedule feed restrictions include: 1) ad libitum food intake (al group), 2) feeding maintenance energy requirement from 7 to 14 d of age 3) feeding to support 50% of the normal ...

The aim of this study was comparing professional futsal practice sessions with and without blood flow restriction on angiogenesis and angiostatin stimulation in active young men. Among physical education students, 12 volunteer selected and randomly divided into two groups of exercise with and without blood restriction. All of the subjects did the 3 vs. 3 professional futsal practice in 6 repeti...

One-hundred eight Mycobacterium avium isolates from pigs, humans, birds, and bovines were typed by the IS1245-based restriction fragment length polymorphism (RFLP) method and PCR-restriction enzyme analysis (PRA) of hsp65. Nine clusters of isolates showing more than 80% similarity in their RFLP profiles were detected. The largest cluster (cluster B) included 32 of 79 pig isolates (40.5%), 3 of ...

background: pregnancies with early onset fetal growth restriction have poor perinatal outcome. sildenafil citrate (pde -5 inhibitor) as a vasodilator increases uteroplacental blood flow and potentiates fetal growth. case presentation: in this study, a case was examined and sildenafil was administered for her. it was found that sildenafil improved the uterine blood flow with a favorable fetal ou...

Iron restriction is the body’s removal of freely available iron from blood and extracellular fluid, as part of the systemic inflammatory response [1]. This process is mediated by hepcidin, which ‘locks’ iron into somatic cells by down-regulating the iron exporter protein ferroportin [2]. Iron accumulates within macrophages, hepatocytes and gut enterocytes, but cannot enter the circulation [1, 2...