conclusions the results of this study phenotypically and genotypically confirmed the high frequency of esbl-producing strains, such as the ctx-m and ctx-m-1 genes, among enterobacteriaceae isolates in our region. therefore, use of antibiotic susceptibility testing for the detection of esbl isolates prior to the prescription of beta-lactam antibiotics is recommended. this could help prevent the ...

background metallo-beta-lactamase (mbl) producing pseudomonas aeruginosa in the burn patients is a leading cause of morbidity and mortality and remains a serious health concern among the clinicians. objectives the aim of this study was to detect mbl-producing p. aeruginosa in burn patients and determine multidrug-resistant (mdr) strains, and respective resistance patterns. patients and methods ...

Introduction of hybrid plasmids, which were constructed by ligation of pCR1 or pMN1 vector plasmid and SalI restriction endonuclease cleaved segments of Streptomyces cacaoi chromosome, resulted in the production of new beta-lactamase and penicillin-binding protein in Escherichia coli. The beta-lactamase and penicillin-binding protein were not from S. cacaoi but rather induced by the plasmids. C...

Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of bla(SHV) gene products gave a "double peak" at position 703, suggesting the presence ...

The stability to beta-lactamase hydrolysis of HR 756, a new cephalosporin antibiotic, was compared to the beta-lactamase stability of cefoxitin and cefuroxime. HR 756, cefoxitin, and cefuroxime were not hydrolyzed by Richmond type I, III, IV, and V beta-lactamases. Antibacterial activity of HR 756 correlated well with resistance to beta-lactamase hydrolysis except against Pseudomonas aeruginosa...

conclusions our study showed high esbl incidence, with ctx-m genotype as the emerging strain in our hospitals. high co-resistance to other non-β-lactam antibiotics is a major challenge for the management of esbl infections. results the esbls were found in (62.89%) of e. coli and (67.5%) of k. pneumoniae isolates. the majority of the typeable isolates harbored two or more esbl genes (73%). overa...

background and objective: acinetobacter baumannii is an aerobic non-motile gram-negative bacterial pathogen that is resistant to most antibiotics. carbapenems are the most common antibiotics for the treatment of infections caused by this pathogen. mechanisms of antibiotic-resistance in a. baumannii are mainly mediated by efflux pumps-lactamases. the aim of this study was to determine antibiotic...

introduction: pseudomonas aeruginosa is one of the leading causes of nosocomial infections, and antibiotic resistance of this pathogen is an important concern in treating such infections. the current work was conducted to investigate the prevalence of bla-imp, and bla-vim metallo-beta-lactamase (mbl) among clinical and environmental p. aeruginosa isolates obtained from icus of different hospita...