Calpastatin (CAST) is a specific inhibiter of Calpains, playing a role in meat tenderization and myogenesis. In the present study the polymorphism of the CAST gene ofKhalkhali goat in Azerbaijan province in Iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (PCR-RFLP). Genomic DNA was extracted from whole blood samples collected from 200 Kh...

background: aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection. in this study, we developed a pcr-single strand confomational polymorphism method to identify the most common aspergillus species and we showed some advantages of this method comparing a pcr-restric­tion fragment length polymorphism with ...

BACKGROUND In order to establish a reliable non-invasive method for sex determination in a bovine fetus in a routine setting, the possibility of identifying specific sequence in the fetal X and Y-chromosomes has been evaluated in maternal plasma using conventional multiplex polymerase chain reaction (PCR) analysis. The aim of this study was to provide a rapid and reliable method for sexing bovi...

Here, we investigated the gene that encodes the main protein component of the byssus in Perna perna (Ppfp) using a consensus fluorescent probe for the Mytilus group and by polymerase chain reaction amplification using specific and random primers. A 524-bp fragment resulting from polymerase chain reaction amplification was found to be homologous to the fp-1 gene of Mytilus coruscus. This fragmen...

among the viruses infecting honey bees, chronic bee paralysis virus (cbpv) is known to induce significant losses in honey bee colonies. cbpv is an unclassified polymorphic single stranded rna virus. using rt-pcr, the virus infections in honey bees can be detected in a rapid and accurate manner. bee samples were collected from 23 provinces of iran, between july-september 2011 and 2012. a total o...

The objective of this study was to evaluate myostatin (MSTN) gene by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) and sequencing methods in four goat breeds including Mohabadi, Markhoz, Lori and Bital breeds. A573 bp fragment of MSTN gene was amplified by PCR. A new 5 bp deletion in 5`UTR (206TTTTA) of the MSTN gene was directly identified by sequencing. The AA...