Ultraviolet B radiation (UVB) is a ubiquitous environmental toxin that causes extensive skin damage resulting in inflammation, photoaging and cancer. UVB known to induce inflammasome activation sterile inflammation associated with cytokine release (e.g. IL-1β, IL-18) immune cell infiltration. We developed circularly permutated luciferase reporters for caspase-1 assess by vitro vivo. HaCaT kerat...

We have cloned and sequenced a chicken homolog to the mammalian interleukin-1beta (IL-1beta)-converting enzyme (caspase-1) cDNA and have evaluated caspase-1 mRNA expression in various tissues from the domestic hen, including ovarian follicle granulosa and theca layers. The deduced amino acid (aa) sequence of chicken caspase-1 is 44.9% identical to human caspase-1, and contains an active site pe...

Alternatively spliced isoforms of certain apoptosis regulators, such as Bcl-x, Ced-4, and Ich-1, have been shown to play opposing roles in regulating apoptosis. Here, we describe the identification of an endogenous alternatively spliced isoform of caspase-9, named caspase-9b, which lacks the central large subunit caspase domain. Caspase-9b is detectable in many cell lines by PCR and at the mRNA...

Abstract BACKGROUND AND AIM Chronic gut inflammation such as inflammatory bowel diseases is thought being associated with neurodegenerative in humans. The direct evidence for and the underlying mechanism of this brain-gut interaction, however, remain elusive. METHODS We used manganese-enhanced magnetic resonance imaging (MEMRI) to assess brain functional activity from awake freely moving mice. ...

We have investigated the ability of Sf-caspase-1 and two mammalian caspases, caspase-1 and caspase-3, to induce apoptosis in Spodoptera frugiperda Sf-21 insect cells. While the transient expression of the pro-Sf-caspase-1 did not induce apoptosis, expression of the pro-domain deleted form, p31, or coexpression of the two subunits of mature Sf-caspase-1, p19 and p12, induced apoptosis in Sf-21 c...