OBJECTIVE To consider the relationship of the parasitic infections including cryptosporidium with chronic diarrhea. Also the effect of chronic disease as pulmonary tuberculosis (TB) and nosocomial infection on the occurrence rate of parasites in cases of chronic diarrhea. METHODS Stool samples were collected from 205 patients in teaching, general, child and maternity hospitals in Basrah, Iraq...

Cryptosporidium, the protozoan parasite, has several transmission routes, including anthroponotic and zoonotic transmission, as well as the foodborne way, but mainly by water. The oocysts, the resistant stage produced by Cryptosporidium, are remarkably stable, and can survive for weeks or even months in the environment. Furthermore, the infective dose is low, probably even a single oocyst can c...

Of 1346 faecal samples from the Chikwawa and Thyolo districts of Malawi, analysed for the presence of Cryptosporidium oocysts between October 2001 and May 2003, 61.3% were from cattle (29.8% of these were from calves <6 months old). Cryptosporidium oocysts were detected during all three seasons studied in Chikwawa and Thyolo. In Chikwawa, 13.6% of adult cattle and 11.7% of calves were infected,...

Purpose: The purpose of this study is to model Cryptosporidiosis in laboratory animals. The parasites were inoculated into animalsand thenmultiplied. The process of proliferation was compared to controlCryptosporidiosis in humans. Materials and Methods: Twenty-five laboratory mice (4-7 days of age) and twenty-five laboratory rats (5 days of age) were assigned to the category I while the cat...

Cryptosporidium and Giardia were characterized in a watershed in southern Ontario, Canada, over a 2½ year period. River samples were collected every two weeks, primarily near a municipal drinking water treatment plant intake. Cryptosporidium and Giardia were frequently detected with an overall occurrence rate of 88 and 97%, respectively. Giardia concentrations were higher than Cryptosporidium, ...

The prevalence of Cryptosporidium infection among lambs and goat kids in Serbia was examined. The animals were grouped according to age, i.e., up to 30 days and from 31 to 90 days. The infection was diagnosed using three coprological procedures. Sheather’s sugar flotation was employed to determine the concentration of oocysts. Modified Ziehl-Neelsen technique and modified Kinyoun technique were...

We demonstrate the application of coherent anti-Stokes Raman scattering microscopy for the rapid, label-free chemical imaging of waterborne pathogens. Chemically selective images of cryptosporidium were acquired in just a few seconds using coherent anti-Stokes Raman scattering microscopy, demonstrating its capability for the rapid detection of cryptosporidium at the single oocyst level. We disc...

To determine the minimum number of Cryptosporidium oocysts that can be detected in stool specimens by diagnostic procedures, stool samples seeded with known numbers of Cryptosporidium parvum oocysts were processed by the modified Formalin-ethyl acetate (FEA) stool concentration method. FEA concentrates were subsequently examined by both the modified cold Kinyoun acid-fast (AF) staining and fluo...

Cryptosporidium is one of the most common causes of childhood diarrhea in developing countries. The aim of this randomized pilot study was to detect and characterize infective species and determine the genotypes of Cryptosporidium parasites in pediatric patients suffering from diarrhea in North West of Iran. A total of 113 fecal samples were collected from diarrheic children hospitalized in Tab...

Giardia and Cryptosporidium levels were determined by using a combined immunofluorescence test for filtered drinking water samples collected from 66 surface water treatment plants in 14 states and 1 Canadian province. Giardia cysts were detected in 17% of the 83 filtered water effluents. Cryptosporidium oocysts, were observed in 27% of the drinking water samples. Overall, cysts or oocysts were ...