We investigated the effect of short-term changes in temperature on alternative (Alt) and cytochrome (Cyt) pathway respiration, both in intact tissues and isolated mitochondria of 14-d-old cotyledons of soybean (Glycine max L. cv Stevens). We also established the extent to which temperature alters the interaction between the oxidizing pathways and the level of ubiquinone (UQ) reduction (UQ(r)/UQ...

Tobacco (Nicotiana plumbaginifolia) seedlings genetically transformed to express apoaequorin were incubated in h-coelenterazine to reconstitute the calcium-sensitive luminescent protein aequorin. Treatment of these seedlings with hydrogen peroxide resulted in a transient burst of calcium-dependent luminescence lasting several minutes. Even though the hydrogen peroxide stimulus was persistent, t...

To study B cell tolerance to the mitochondrial protein cytochrome c (CYT), the B cell response to pigeon CYT (PCC) was examined in mice transgenic for PCC. PCC was coupled to OVA to provide T cell help, since PCC-specific T cells in PCC-transgenic mice are deleted in the thymus. The frequency of secondary B cells responding to the minor antigenic surface around residue 44 on PCC was decreased a...

Rollers crimpers have been used in conservation agriculture to terminate cover crops; however, excessive vibration generated by the original straight-bar roller design has delayed adoption of this technology in the United States. To avoid excessive vibration, producers generally reduce operating speeds that increase the time needed to perform the field operation. The objectives of this research...

and Implications The aim of this study was to test if further grinding of corn DDGS is advantageous in terms of apparent total tract digestibility (ATTD) of DM, GE, nitrogen (N), and NDF in growing and finishing pigs. It was also tested to determine if ATTD was different between hammermill and roller mill grinding methods. There were 3 diets. Each diet consisted of 51% corn ground at 500 micron...