Newcastle disease (ND) is one of the contagious viral diseases in avian species. Recently, several ND outbreaks in pigeon caused by pigeon paramyxovirus serotype-1 (PPMV-1) have been reported in limited numbers from Iran and phylogenetic studies have been conducted on partial sequence of NDV fusion (F) gene. In the present study, ten PPMV-1, named Pigeon_paramyxovirus1_isolate_pigeon/Iran/UT_EG...

background: the double heterozygous state of α/β thalassemia may alter the hematological indices and modify the phonotype. in addion, definite characterizaon of co-inheritance of α- and β-thalassemia heterozygous carriers may change the process of genec counseling. materials and methods: an iranian couple with low hematological indices was analyzed for α-globin gene deleons using mulplex g...

Background: Many plant growth-promoting bacteria including Rhizobia contain the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme that can leave ACC, and thereby lower the level of ethylene in stressed plants. Drought and salinity are the most common environmental stress factors for plants in Iran. Objectives: The main aim of this research was development of bio-fertilizers containing A...

abstract       background and objective: hepatitis b virus (hbv) is a dna virus with high tendency toward hepatic tissue. there are currently about 3 million hbv-infected people and 350 to 400 million chronic carriers of this virus in the world. x protein plays a role in the over-expression of oncogenes, carcinogenicity of liver cells and overlaps with the basal core promoter of the virus. muta...

The lpsA gene, a late acting gene in the biosynthetic pathway of ergovaline, a suspected causative agent for fescue toxicosis in cattle, has been cloned from Neotyphodium lolii, an endophytic fungus of Lolium perenne. In this study, a similar gene was detected in several strains of endophytic Neotyphodium spp. isolated from grass hosts endogenous to Iran using direct and nested-PCR assays. Exce...

four exons of the human genomic gm-csf gene were assembled together using gene splicing by overlap extension (soe) method. the resulting nucleotide sequence was cloned in the pet23a(+) ex pression vector under the control of strong bacteriophage t7 transcription and translation signals. the construct obtained was transferred into the e. coli strain, bl21(de3) plyss and iptg was used for inducti...

phenylketonuria (pku) is an inborn error of amino acid metabolism with an autosomal recessive inheritance caused in most cases by mutations in the phenylalanine hydroxylase (pah) gene. pku has wide allelic heterogeneity. here we report a novel heterozygous substitution (c.1223g>t (p.arg408leu)) in the pah gene in an iranian pku family. the patient was 19-yr-old female with diagnosis of moderate...

abrin known as ribosome inactivating protein’s (rip) it is a high cytotoxic plant protein. high lethality and low cost and easy access to plant and seeds lead to this toxin used in crimes and terrorist acts. because obtaining purified toxins requires advanced laboratory equipment and complex procedures, so, it appears that the perpetrators of such crimes use crude extracts. as a result, remaini...

objective(s):brucellosis is a well-known domestic animal infectious disease, which is caused by brucella bacterium. groel antigen increases brucella survival and is one of the major antigens that stimulates the immune system. hence, the objective of the present study was cloning and bioinformatics analysis of groel gene. materials and methods: the full-length open reading frame of this gene was...

objective(s) recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. in the present experiment, a reco...