BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Low pH triggers the translocation domain of diphtheria toxin (T-domain), which contains 10 α helices, to insert into a planar lipid bilayer membrane, form a transmembrane channel, and translocate the attached catalytic domain across the membrane. Three T-domain helices, corresponding to TH5, TH8, and TH9 in the aqueous crystal structure, form transmembrane segments in the open-channel state; th...

This study is the result of an attempt to investigate the differences between the Persian translated drama text (page) of each English drama text with its performance on the stage (stage) in Iran. In other words, the present researchers tried to find the implemented changes in page which make it real on the stage in the target language and culture in order to show that in drama translating and ...

Methyltransferase RlmJ uses the cofactor S-adenosylmethionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli. RlmJ with a C-terminal hexahistidine tag was overexpressed in E. coli and purified as a monomer using Ni(2+)-affinity and size-exclusion chromatography. The recombinant RlmJ was crystallized using the sitting-drop vapo...

Virtual reality drama has the benefit of enhancing immersion, which was lacking in original e-learning systems. Moreover, dangerous and expensive educational content can be replaced by stimulating users’ interest. In this study, we investigate the effects of avatar performance in virtual reality drama. The hypothesis that the psychical distance between virtual characters and their viewers chang...

Socrates and Meno reach two different conclusions: in the first part of the dialogue, that virtue is knowledge and can therefore be taught; in the second, that it is reliable true opinion and can therefore be acquired only by divine inspiration. Taking into account Socrates’ role as a teacher (of his interlocutors and of Plato) and Plato’s role as a teacher (of us), I show that neither of these...