نتایج جستجو برای: specific primers acetyl
تعداد نتایج: 1075127 فیلتر نتایج به سال:
Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and seque...
Background and Aims: Mycoplasma pneumoniae is one of the most important pathogens causing human respiratory tract infection; especially in community-acquired pneumonia (responsible for 10 – 40% of these infections).The aim of this study was to evaluate the prevalence of Mycoplasma pneumonia in patients with respiratory infections from Mostafa Khomeini and Khatam hospitals, by culture and molecu...
Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only cursory or even no validation. To directly examine the efficacy and specificity...
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under considera...
Background and purpose: Polymerase chain reaction (PÇR) is a rather quick and accurate method employed for gene detection and isolation. Primer designing is an important issue in this technique and plays a critical role in considering both the genome properties and cloning of the isolated genes. Streptomycin antibiotic is produced by Streptomyces griseus using str gene cluster with more than 25...
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