Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grünwald-Giemsa) and DNA-spec...

An 18 year old female presented with generalized maculopapular rash and headaches. The skin and bone marrow biopsy revealed numerous mast cells which stained metachromatically with giemsa and toludine blue stains and showed positivity with CD68 and CD117 on immunohistochemistry. Her clinical presentation and laboratory findings were consistent with the diagnosis of indolent systemic mastocytosi...

BACKGROUND Cutaneous Leishmaniasis (CL) is a parasitic skin disease. Diagnosis primarily is based on clinical signs and microscopic observation of parasite on direct stained smears or tissue sections. Sensitivity of direct smear is not as high as molecular methods. The aim of this study was to identify and characterize Leishmania species among the negative direct smears obtained from skin ulcer...

Aim: Helicobacter pylori infection has been ascertained to play pivotal role in the pathogenesis of chronic gastritis and gastric neoplasia.1The present study was performed evaluate diagnostic accuracy H&E stain Giemsa for histological diagnosis helicobacter by taking immunohistochemical staining as a gold standard. Methods: A total 155 cases were included our study. The received biopsies f...

Background: Theileria annulata is predominant and of utmost economical importance tick borne pathogen bovines in the region which routinely diagnosed based on microscopic examination Romanowsky stained thin blood smears. Present study was intended to evaluate analyze detection efficacy a commercial polymerase chain reaction kit assay comparison conventional PCR classical microscopy for T. from ...