Callus tissues induced from shoots of species in the orange subfamily (Aurantioideae) were grafted in vitro to evaluate the correlation between reaction at the callus graft interface and taxonomic relationship. The graft interface between two pieces of callus from species that are taxonomically close was not distinguishable by anatomical observations. On the other hand, in the combinations of l...

To develop an ef®cient procedure for plant regeneration from leaf-derived callus of Lavandula vera DC, the production of multiple shoots and the formation of roots from these shoots were studied. When calli were cultured in a medium with 4.0 10ÿ7 M N-(2-chloro-4-pyridyl)-N0phenylurea (CPPU), urea-type cytokinin, multiple shoots were obtained from the greenish surface of the callus ef®ciently at...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

Pollen plants from anther culture in rice breeding allows selection of fine strains in early generations. Exploitation of anther culture technique in research is limited due to very low regeneration frequency of indica cultivars and the transfer of anther culture traits from japonica to indica varieties is important in improving these rice varieties. In this study the optimization of media requ...

An active and tetrameric form of recombinant butyrylcholinesterase (BChE), a large and complex human enzyme, was produced via semicontinuous operation in a transgenic rice cell suspension culture. After transformation of rice callus and screening of transformants, the cultures were scaled up from culture flask to a lab scale bioreactor. The bioreactor was operated through two phases each of gro...

Barley is an important cereal crop worldwide. Its genetic transformation now limited to very few cultivars because of the high genotype dependence embryogenic callus. To reveal key genes or factors controlling callus induction and plantlet regeneration in barley, we compared transcriptomic profiles immature embryos Golden Promise ZU9, which differed dramatically efficiency transformation. The s...

The cactus Mammillaria gracillis Pfeiff. was propagated in vitro on Murashige and Skoog (MS) medium without any growth regulators. At the bases of some plants abundant callus masses developed. Lower agar and higher MS salt concentrations stimulated callus pro­ duction. In the culture, morphologically normal and hyperhydric shoots were regenerated. The growth of crown-gall tumour was induced on ...

The establishment of cell suspension culture from the callus of sugar beet (Beta vulgaris L.) breeding lines and plant regeneration from suspension-derived callus are described in this study. Using different concentrations and combinations of BAP and 2,4-D, the growth patterns of cell suspension cultures were examined during a range of culture periods (0, 3, 5, 7, 9, 11, 13 and 15 days). In all...

A simple method was developed to permit albino plant regeneration from anther culture of Hobigonj Boro (Hbj B) IV and Hbj B VI, two local varieties of aromatic indica rice from Bangladesh. Three crucial factors were identified for the albino shoot primordia to change into green plantlets in culture; components of M10 induction medium, callus size (range 0.2-0.4 cm long) and height of shoot prim...