background: iron is an essential trace element in cell proliferation. several investigations demonstrate that iron deprivation inhibits cell proliferation. however, the impact of iron on telomerase activity of activated lymphocytes remains unexplained to date. objective: in this study, the effect of iron on the proliferation and telomerase activity of lymphocytes stimulated by phytohemagglutini...

conclusions the pc-ns3 possesses the capacity to express ns3 in the mammalian cell line and demonstrated strong immunogenicity in a murine model. our primary results demonstrated that the immunogenic truncated region of ns3 could be used as a potential vaccine candidate against hepatitis c. results the pcdna3.1 plasmid harboring the coding sequence of ns3 (pc-ns3) was constructed and confirmed ...

a multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (sf) of irradiated cells. the estimation of sf based on the cell‑growth curve information is the major advantage of this assay. in this study, the utility of multiple‑mts assay for the sf estimation of irradiated ht‑29 colon cancer cells, which were plated before irradiation, w...

Objective(s):Breast cancer is an important leading cause of death from cancer. Stathmin and tau proteins are regulators of cell motility, and their overexpression is associated with the progression and bad prognosis of breast cancer. Memantine, an N-methyl-D-aspartate (NMDA) receptor antagonist, is the potential inhibitor of tau protein in neurons. This study determines the effect of memantine ...

conclusions the proliferation and invasion of human bladder cancer cells can be inhibited by rnai-targeting skp2. as a result, skp2 may be a potential target for gene therapy in cases of human bladder cancer. materials and methods the expression of the skp2 gene was knocked down by rna interference (rnai) in t24 cells. the transcription level of skp2 was detected by reverse transcription-quanti...

Marine microorganisms are important sources for novel natural products. Hence, the aim of this study was introducing the marine microorganisms with capability to produce antioxidant and cytotoxic metabolites. For this purpose, ten live sea cucumbers (Holothuria leucospilota) were collected from Larak Island. Then, their intestine contents were serially diluted and treated by heating (55 °c). 10...

Background: Human colorectal cancer cells overexpress carcinoembryonic antigen (CEA). CEA is a glycoprotein which has shown to be a promising vaccine target for immunotherapy against colorectal cancer. Objective: To design a DNA vaccine harboring CEA antigen and evaluate its effect on inducing immunity against colorectal cancer cells in tumor bearing mice. <str...

Objective(s): Hepatocellular carcinoma (HCC) is one of the leading fatal neoplasms and the most common primary liver malignancy worldwide. Peptide hormone ANGPTL8 (betatrophin) may act as an important regulator in HCC development through the Wnt/β-catenin pathway. We aimed to evaluate the effects of recombinant ANGPTL8 on Wnt/β-catenin signaling in human liver carcinom...