BACKGROUND Both Helicobacter pylori genotype and host genetic polymorphisms play a role in determining the clinical consequences of H. pylori infection. We investigated whether there are any combinations of bacterial and host genotypes that are particularly associated with the occurrence of gastric carcinoma. METHODS Genotypic variations in virulence-associated genes of H. pylori vacA (s and ...

Polymorphisms in IL-1 gene have been suggested to influence transcription of IL-1α and IL-1B and thereby the pathophysiology of periodontitis. Using genotyping IL-1 test, a pilot study was conducted on 32 Syrian patients with aggressive periodontitis (AgP) and 35 healthy controls to investigate the association between the IL-1α(-889), IL-1B(+3953) gene polymorphisms and AgP among schoolchildren...

Martin, Christian, Andrea Wohlsen, and Stefan Uhlig. Changes in airway resistance by simultaneous exposure to TNF-a and IL-1b in perfused rat lungs. Am J Physiol Lung Cell Mol Physiol 280: L595–L601, 2001.—Tumor necrosis factor (TNF)-a and interleukin (IL)-1b are formed simultaneously under inflammatory conditions such as asthma and acute respiratory distress syndrome. Here we investigated the ...

The IkB kinases (IKKs) lie downstream of the NF-kBinducing kinase (NIK) and activate NF-kB by phosphorylation of IkBa. This leads to IkBa degradation and release of NF-kB. In U937 monocytic cells, interleukin (IL)-1b (1 ng/ml) and tumor necrosis factor (TNF)-a; 10 ng/ml) induced kB-dependent transcription equally. However, IKK activity was strongly induced by TNF-a but not by IL-1b. This was co...

To further understand the molecular mechanism of glucocorticoid action on gene expression, DNA-binding activities of the cis-acting transcription factors activator protein 1 (AP1), AP2, Egr1 (zif268), NF-kB, the signal transducers and activators of transcription proteins gamma interferon activation site (GAS), Sis-inducible element, and the TATA binding protein transcription factor II D (TFIID)...

Macrophage is the immune phagocytic cell, playing variety of immunological and inflammatory reactions. The macrophage activation has been extensively studied in vitro using culture media like Dulbecco's modified Eagle’s medium (DMEM) Ham's F-12 (F-12) response to bacterial infection, tumor development, cytokines so on. In study during co-culture with EL-4 cells, we found different phenotypes J7...

present investigation was carried out to identify dna polymorphism of il-2rγ and chb6 genes. sixty five birds belonging to the indigenous chicken of himachal pradesh were utilized. good quality dna samples were subjected to pcr-rflp analysis using chicken specific primers. overnight restriction enzyme digestion was carried out at 37°c with iu hph i and pvu ii for il-2rγ and chb6 genes, respecti...

Long COVID, also referred to as Post-Acute Sequelae of COVID (PASC), is probably triggered during SARS-CoV-2 infection and acute COVID-19 by Spike-protein binding hyper-activating the cell-membrane expressed Receptor for Advance Glycation End-products (mRAGE) Toll-Like 4 (TLR4). infects lung monocytes Spike mRAGE (not ACE2). During COVID-19, high levels IL-6 hyper-stimulate S100A8/A9 expression...

Eukaryotic translation initiation factor 4E (eIF4E) has been known to play a critical role in the regulation of gene expression eukaryotes and affect many essential cellular processes, including proliferation, apoptosis differentiation. We others found eIF4E other eIF subunits upregulated human psoriatic skin thus hypothesized that they are involved pathophysiology psoriasis can serve as direct...

Interleukin-1β (IL-1β) has a role in β- cell destruction in autoimmune diabetes by stimulating the expression of inducible nitric oxide synthase (iNOS) that generates the free radical nitric oxide. We aimed to investigate the effect of Achillea millefolium L, as a traditional hypoglycemic agent, on IL-1β and iNOS gene expression of pancreatic tissue in the STZ- induced diabetic rats. Forty adul...