Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...

background: hepatitis g virus (gbv-c) is a single strand rnapositive virus and is a member of flaviviridae family. infection with the virus is prevalent add has worldwide distribution. accurate diagnosis of the virus is very important, because of co-infection with other important viruses like hcv, hbv and hiv, it may influence on pathogenesis, disease progression and response to viral therapy. ...

conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

abstract beet yellows virus (byv), the type member of the genus closterovirus and one of the most economically important yellowing viruses, causes serious losses in sugarbeet. in order to study infection and distribution of beet yellows virus during summer 2007, about 530 samples were collected from beet’s fields in razavi khorasan province (fariman, torbate jam, ghoochan, chenaran, torbate hey...

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...