Background: Coronavirus disease 2019 (COVID-19) is still causing disastrous effects in various parts of the world through recurring waves. Real-time reverse transcription polymerase chain reaction (RT-PCR)-negative COVID-19 particularly challenging as these patients are less likely to receive treatment and more progress severe disease. Thus, it imperative find markers that can predict severity ...

Purpose: Prove that Computed Tomography (CT) is a more effective diagnostic tool for diagnosing Coronavirus Disease 2019 (COVID-19) in comparison with Reverse Transcription Polymerase Chain Reaction (RT-PCR) due to its higher sensitivity.
 Methods and materials: A total of 200 cases had records chest CT scans RT-PCR tests were collected from Palestinian governmental hospitals September Nov...

INTRODUCTION Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE). METHODS The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program. RESULTS Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectiv...

Abstract Background Hypertrophy is a critical process for chondrocyte differentiation and maturation during endochondral ossification, which responsible the formation of long bone postnatal longitudinal growth. Increasing evidence suggests that melatonin, an indole hormone, plays pivotal role in chondrogenesis. However, little known about effects melatonin on terminal chondrocytes. Methods Mese...

the p75 pan-neurotrophin receptor (p75ntr) plays a pivotal role in linking the immune system with the nervous system. p75ntr is required for the development of several characteristic features of allergic asthma. also p75ntr upregulated by reactive schwann cells after peripheral nerve injury. moreover p75ntr and rhoa play a critical role in the regulation of apoptosis. to determine whether the d...

Real-time reverse transcription polymerase chain reaction (RT-PCR) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are occasionally repeated when clinicians suspect false-negative results, but the conditions under which RT-PCR testing is warranted remain unclear. We evaluated practice of repeat SARS-CoV-2 in 45 patients who were retested after an initial negative PCR test...

Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs cation step adds time, cost, and aff ects the quality of testing. objective this study was to develop a protocol for direct saliva in genetic markers, without acids. Methods. PCR, real-time RT-PCR isothermal amplifi were used detection Results. We report markers ...

BACKGROUND & OBJECTIVES Traditionally, rabies diagnosis is made by demonstration of rabies viral antigen by direct immunofluorescence (DIF) and mouse inoculation test (MIT). The present study was carried out to evaluate the role of reverse transcriptase-polymerase chain reaction (RT-PCR) in comparison with these conventional techniques for the diagnosis of rabies. METHODS Skin biopsies, corne...