Background: Conventional anti-cancer treatments, such as surgery, chemotherapy, radiation therapy, etc., are linked to antimicrobial resistance (AMR) and have negative effects on healthy tissues. Objectives: The flow cytometry technique was used in this study to evaluate the effectiveness of a modified anti-cancer peptide in treating the AGS gastric cancer cell line. Methods: This study used ...

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

Background Over the last few decades, several studies have shown that plants can be a viable option for producing functional recombinant proteins with a wide range of structural characteristics [1]. In addition, the potential benefit for developing countries is a prominent feature that we have recently addressed [2]. Plant-produced recombinant proteins can already be considered a novel componen...

Nerve growth factor (NGF) is a neurotrophic factor that is functional in the survival, maintenance and differentiation of nervous system cells. This protein has three subunits, of which the beta subunit has the main activity. Its structure consists of a cysteine knot motif made up of beta strands linked by disulfide bonds. It can be used as a therapeutic agent in the treatment of many diseases....

Outer membrane proteins (OMPs) of Pasteurella multocida play an important role in virulence and are for vaccine development due to immunogenicity. Here, we evaluated immunoprotection mice, immunized with native OmpH, recombinant OmpH HS alum precipitated P. P52 against challenge. Protection studies were performed mice groups I (OmpH); II (recombinant OmpH); III (HS vaccine). Group IV was contro...

conclusions we have highlighted the role of rns3 plus rnt-gp96 mediated by α5integrin in producing il-12 and tnfα. it can be suggested that rnt-gp96 could enhance immunity characteristic of rns3 protein via production of pro-inflammatory cytokines. results our results showed that rnt-gp96 alone significantly increases the expression level of il-12, tnfα and α5integrin in thp-1 macrophages and d...

A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...

Background: The expression of bio-therapeutic proteins in mammalian cells, such as CHO, attains high homogeneity related to post-translational modifications. Although CHO remains the most popular cell line for bestselling biotherapeutic proteins on the market, there are still drawbacks such as expensive culture media, long time line, and high drug cost. Recently, researches on a novel Leishmani...