Dental decay is a disease that is greatly affected by environmental components, but recently there have been an increasing number of documents supporting a genetic factor in the development of caries. The purpose of this study was to examine the association between dental caries and single-nucleotide polymorphisms in the AMELX gene. This research was carried out on 360 individuals of both sexes...

BackgroundIt has been confirmed that the connective tissue growth factor (CTGF) gene rs9402373 polymorphism is associated with fibrotic and inflammatory diseases. However, studies on relationship between polymorphisms in CTGF bowel disease (IBD) remain rare. Therefore, aim of this study was to assess association IBD susceptibility a Chinese population.Materials methodsTo establish an amplificat...

data not shown). Figure 1 demonstrates the two alleles of exon 15 of hMLH1 obtained with and without the modifications described above. The modified technique resulted in unequivocal resolution in the complete absence of artifactual bands. Representative samples of hMLH1 exon 15 were subcloned (TA Cloning® Kit; Invitrogen, San Diego, CA, USA) and sequenced following manufacturer’s instructions ...

To develop a physical map, one often uses a large-insert library to establish a contiguous set of overlapping genomic clones (‘contig’). One of the most efficient ways to prepare contigs in limited chromosomal regions is chromosome-walking. For this approach, it is important to retrieve DNA sequences from the termini of the insert fragment to screen for overlapping clones. Many methods for the ...

this study was conducted in order to investigate the association between the single nucleotide polymorphism (snp) rs2305957 g/a and recurrent pregnancy loss (rpl) in a group of palestinian women residing in gaza strip. a retrospective case-control study was carried out during the period of may to august 2015. a total of 380 females including 190 recurrent pregnancy loss (rpl) patients and 190 c...

We have developed a new package of computer programs and algorithms for different PCR applications, including allele-specific PCR, multiplex PCR, and long PCR. The package is included in the upcoming VectorNTI suite software and attempts to incorporate most of the current knowledge about PCR primer design. A wide range of primer characteristics is available for user manipulation to provide impr...