Gene products that are only expressed in one tissue or cell type are useful models for investigating the biochemical and molecular mechanisms of tissue/cell-specific gene regulation. The regulatory regions of such genes are also practical tools in gene therapy. In this work, prostate-specific and androgen-dependent gene regulation was investigated by using human prostatic acid phosphatase (hPAP...

Abstract Intracellularly active complement C3 and C3a emerged as central regulators of normal immune cell function proliferation via control mTOR glycolysis. Because increased glycolysis is a hallmark many cancer cells, we hypothesized that augmented cell-intrinsic activity may contribute to epithelial malignancy. However, while benign prostate cells (PECs) harbored expected intracellular C3/C3...

Prostate cancer is the most common cancer in males and the second leading cause of cancer deaths in western countries. Although most prostate cancers initially respond well to androgen withdrawal, they become inevitably resistant to this treatment and progress to hormone-insensitive disease after only a few months or years. The therapeutic options in these advanced tumors are very limited. The ...

Alanine scanning mutagenesis of a recently reported prostate cancer cell-selective Protein Transduction Domain (PTD) was used to assess the specific contribution each residue plays in cell uptake efficiency and cell-selectivity. These studies resulted in the identification of two key residues. Extensive mutagenesis at these key residues generated multiple mutants with significantly improved upt...

nloaded GRP3 mediates the activation of the Ras signaling pathway that is present in many human cancers. Here, lored the involvement of RasGRP3 in the formation and maintenance of the prostate cancer phenotype. P3 expression was elevated inmultiple human prostate tumor tissue samples and in the human androgenndent prostate cancer cell lines PC-3 and DU 145 compared with the androgen-dependent p...

Objective(s): The stress-responsive genes of Sestrin family are recognized as new tumor suppressor genes in breast carcinoma, however, the function of Sestrin family in human prostate cancer is not clear. Ionizing radiation (IR) is known to induce Sestrin gene expression in breast cancer cells. However, the response of Sestrin to IR has not been reported in PC3 prostate cancer cells. Materials ...

ownload GRP3 mediates the activation of the Ras signaling pathway that is present in many human cancers. Here, lored the involvement of RasGRP3 in the formation and maintenance of the prostate cancer phenotype. P3 expression was elevated inmultiple human prostate tumor tissue samples and in the human androgenndent prostate cancer cell lines PC-3 and DU 145 compared with the androgen-dependent p...

Downlo GRP3 mediates the activation of the Ras signaling pathway that is present in many human cancers. Here, lored the involvement of RasGRP3 in the formation and maintenance of the prostate cancer phenotype. P3 expression was elevated inmultiple human prostate tumor tissue samples and in the human androgenndent prostate cancer cell lines PC-3 and DU 145 compared with the androgen-dependent pr...

AIM To explore the effects of PCK3145 beyond prostate cancer. MATERIALS AND METHODS Using Trypan blue, MTT proliferation assays, cell cycle and apoptosis analysis, we assessed the effects of PCK3145 on prostate (PC-3), breast (MCF-7) and colon (HT-29) human cancer cell lines and in osteosarcoma (MG-63) cells; any synergistic effects with docetaxel and oxaliplatin were also explored. RESULTS...