نتایج جستجو برای: soluble protein adult stage
تعداد نتایج: 1861660 فیلتر نتایج به سال:
the aim of the present study was to determine the in?uence of presence or absence of corpus luteum (cl) on hormonal and metabolites composition of follicular ?uid (ff) harvested from different sized follicles and its relationship with blood serum concentrations in sanjabi ewes. ovaries and blood samples were collected from 60 clinically healthy adult ewes (sanjabi breed) 1–3 years of age in dio...
Background: Recombinant proteins overexpressed in E. coli are usually deposited in inclusion bodies. Cysteines in the protein contribute to this process. Inter- and intra- molecular disulfide bonds in chitinase, a cysteine-rich protein, cause aggregation when the recombinant protein is overexpressed in E. coli. Hence, aggregated proteins should be solubilized and allowed to refold to obtain nat...
Background: The production of recombinant proteins in Escherichia coli is one of the most valuable achievements in biotechnology, with many therapeutic and diagnostic applications; however, the aggregation and misfolding of proteins that result in the formation of insoluble inclusion bodies is a disruptive factor in this process. Various solubilization and refolding methods can be used to impro...
Glutamyl ribonucleic acid synthetase was purified approximately 150-fold from homogenates of rat liver to a specific activity of 60 mpmoles of glutamyl-RNA formed per min per mg of protein. The enzyme at this stage of purification was judged to be about 40% pure, but in a state of aggregation. Although the enzyme was quite stable to storage, it was very heat labile, and lost activity during its...
In order to evaluate the effect of Inoculation by pseudomonas plant growth promotinh rhizobacteria and foliar application of boron-nanochelate of pea( Pisum sativum L.) mother plants on germination traits and seedling growth of produced seeds under Cadmium stress a factorial experiment based on completely randomized design with four replications was conducted. Experimental treatments were 12 c...
Silver ion and hydrogen peroxide act synergistically as a strong disinfectant. The purpose of this study was to assess the effect of combined hydrogen peroxide 50% and silver ion 0.05% at four life stages of Indian white shrimp (Fenneropenaeus indicus) and to evaluate the feasibility of using this substance in shrimp culture. The Trimmed Spearman-karber software was applied for determining EC...
Spleen cells of mice vaccinated with radiation-attenuated Schistosoma mansoni cercariae were used to produce monoclonal antibodies directed against newly transformed schistosomular surface antigens. One of these monoclonal antibodies recognized a polypeptide of 18 kDa. This glycoprotein was purified by monoclonal antibody immunoaffinity chromatography and a polyclonal antiserum was prepared aga...
background and objectives: fast proteolysis of cheese in ripening process may lead to the premature attack of casein, release of the majority of enzymes into the whey, and loss of cheese composition from curd to whey. in this study, the effect of liposomal flavourzyme on proteolysis of iranian white brined cheese, as well as on the yield and composition of whey and curd was investigated. materi...
Verticillium dahliae Kleb.is a soilborne pathogen that cause vascular wilt in chrysanthemumplant (Dendranthema grandiflorum (Ramat.) Kitam). The objective of this research was to study the levels of some organic solutes, such as total protein, total soluble sugars, starch and proline, relative water content, RNA level, malondialdehyde and H2O2 contents in leaves of chrysanthemuminoculated with ...
Genotype choosing and cutting stage are very significant to obtained better forage yield quality. However, researches about production from quinoa plant genotypes harvested at different stages not enough in Mediterranean conditions. This study was conducted determine the quality of five (Red head, Cherry vanilla, French Mint vanilla Titicaca) three (flowering, milky dough). The experiment laid ...
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