In contrast to peas (Pisum sativum), where mitochondrial lipoamide dehydrogenase is encoded by a single gene and shared between the alpha-ketoacid dehydrogenase complexes and the Gly decarboxylase complex, Arabidopsis has two genes encoding for two mitochondrial lipoamide dehydrogenases. Northern-blot analysis revealed different levels of RNA expression for the two genes in different organs; mt...

In this communication we describe serum-free culture conditions for the serial propagation of the C6 glioma cell line. The growth rate, saturation density, and morphology of these cells are equivalent to those of their serum-grown counterparts when cultured in a 3:1 mixture of Dulbecco's modified Eagle's medium and Ham's medium F-12 supplemented with trace elements, insulin, transferrin, fibrob...

Substitute valuations (in some contexts called gross substitute valuations) are prominent in combinatorial auction theory. An algorithm is given in this paper for generating a substitute valuation through Monte Carlo simulation. In addition, the geometry of the set of all substitute valuations for a fixed number of goods K is investigated. The set consists of a union of polyhedrons, and the max...

Substitute valuations (in some contexts called gross substitute valuations) are prominent in combinatorial auction theory. An algorithm is given in this paper for generating a substitute valuation through Monte Carlo simulation. In addition, the geometry of the set of all substitute valuations for a fixed number of goods K is investigated. The set consists of a union of polyhedrons, and the max...

There are occasional reports of using the appendix to replace sections of the ureters, and to our knowledge, only a couple of them were in the left side. We used the appendix as a ureteral substitute in two cases in technically difficult parts, the upper left ureter and the first part of the right ureter. Follow-ups of8 years and 6 months in right and left side cases, respectively, are imp...

Ring Opening Polymerization In article 2200359, Sonja Herres-Pawlis, Andreas Jupke, and co-workers show that the transition of new non-toxic catalysts for lactide ring opening polymerization from lab scale to industry is a highly complex process. Hence, promising end up in valley death between these scales. Model-based analysis can bridge this gap, giving an in-depth understanding pave way subs...

The plastid ClpPR protease complex in Arabidopsis thaliana consists of five catalytic ClpP and four noncatalytic ClpR subunits. An extensive analysis of the CLPR family and CLPP5 is presented to address this complexity. Null alleles for CLPR2 and CLPR4 showed delayed embryogenesis and albino embryos, with seedling development blocked in the cotyledon stage; this developmental block was overcome...

We give an algebro-geometric construction of the Hitchin connection, valid also in positive characteristic (with a few exceptions). A key ingredient is substitute for Narasimhan-Atiyah-Bott K\"ahler form that realizes Chern class determinant-of-cohomology line bundle on moduli space bundles curve. As replacement we use explicit realisation Atiyah this bundle, based theory trace complex due to B...

Functional chaperone cooperation between Hsp70 (DnaK) and Hsp104 (ClpB) was demonstrated in vitro. In a eubacterium Thermus thermophilus, DnaK and DnaJ exist as a stable trigonal ring complex (TDnaK.J complex) and the dnaK gene cluster contains a clpB gene. When substrate proteins were heated at high temperature, none of the chaperones protected them from heat inactivation, but the TDnaK.J comp...