BACKGROUND Helicobacter pylori, which is associated with many upper gastrointestinal diseases, is found in half of the population of the world. Several special stains and immunohistochemistry stain for H. pylori are available. The need for and usefulness of immunohistochemical (IHC) technique has been debated for years. Toluidine blue is a simple stain for microbiological studies and is easily ...

Objective: to know and document the risk of factors for category leishmaniasis. Material and Methods: Cutaneous leishmaniasis is rapidly and easily diagnosed by direct examination of impression smear from lesion. Smear was stained by giemsa or Wright stain. In active lesion amastigote form of parasite are easily recognized intracellularly and extracellularly. Diagnosis of visceral leishmaniasis...

background and objectives: anaplasma phagocytophilum is a zoonotic, tick borne rickettsial pathogen. a. phagocytophilum has been detected in north america, europe, africa and asia by molecular methods. in iran we have little information about the distribution of this agent in human and animals. materials and methods: from march 2007 to july 2007, one hundred and fifty blood samples and correspo...

Giemsa-stained blood smears are mainly used for microscopic examination to diagnose malaria. However, they may also be subjected to polymerase chain reaction (PCR) to confirm diagnosis or for retrospective studies requiring the analysis of old smears. We investigated the possibility of DNA contamination occurring during automated Giemsa staining or due to the failure to clean the oil-immersion ...

To determine the volume of bronchoalveolar lavage (BAL) fluid necessary to diagnose Pneumocystis carinii pneumonia (PCP) in immunocompromised patients, specimens from 25 patients were evaluated. Twenty-one patients were HIV infected. BAL was performed using three to four 60-mL aliquots of room temperature, sterile, saline solution. Each syringe of BAL effluent was numbered and its volume was me...

This study concerns a comparative analysis of the acridine orange and Giemsa staining procedures for the fish erythrocyte micronucleus assay. The goal was to optimize the assay in the context of field water monitoring. Fish (Carassius carassius) were exposed to a reference genotoxic agent, cyclophosphamide monohydrate 5 mg l(-1) for 2, 4, and 6 days before testing. Slides from each individual w...

The morphology of Trypanosoma cruzi is reviewed since the initial description of Giemsa-stained preparations by Carlos Chagas until the most recent micrographs obtained with freeze-fracture techniques. Special emphasis is given to structures such as the cell surface, the flagellum, the kinetoplast, the reservosomes and the endocytic pathway, and the acidocalcisomes.

I N THE COURSE of cunrrent investigations on leukocyte granules, the questioni arose as to w’hether the group responsible for the stable sudanophilia might be a primary amine. It is well known that primary amines are differentially destroyed by nitrous acid,’3 thus: RNH2 + HNO2-ROH + H2O + N2. In testing this hypothesis we used both Vans Slyke’s reagent* arid molar solutionis of sodium nitrite ...