objective: organophosphorus hydrolase (oph) is a homodimeric enzyme that can hydrolyze phosphoester bonds and reduce the toxicity of organophosphorus compounds. this makes oph a suitable element for the biodegradation of these compounds. methods: we successfully cloned the oph gene from pseudomonas diminuta, after optimization for pichia pastoris, into a yeast expression vector (ppiczαb). afte...

this experiment was designed to evaluate the effects of different levels of tomato pomace (dtp) with different multi enzymes on the performance and egg quality of laying hens at the first phase of production. in this experiment 432 hens were used in a completely randomized design in a factorial arrangement of 4×4, with four levels of tomato pomace (0, 4, 8 and 12) and different enzymes (without...

a total of 288 broiler chickens (1 d old) were used in a 42-d growth trial to study the effects of chicory (cichorium intybus) and nettle (urtica dioica) with or without multi-enzyme on growth performance, carcass traits, and biochemical parameters. the corn-soybean based control diet and 8 experimental diets were fed to each of 3 pens of chickens (12 chickens/pen). treatments were as follows: ...

conclusions allicin vapor, or some decomposition product of allicin, causes inhibition of bacterial growth and biofilm formation. parallel enzymatic studies confirmed this inhibitory effect and suggest that sulfhydryl enzymes are involved in biofilm formation in the strain studied. results an inhibition effect was observed both in the bacterial cultures and the enzymatic solutions, and the exte...

ziziphus spina-christi are distributed in arid and semi-arid regions of world. most of these areas are subjected to soil salinity. so, the aim of this study was to find out the effects of different nacl concentrations on z. spina-christi seedlings growth, in the presence of a number of single and dual amf inoculums, to provide some information about possible effects of amf under salinity condit...

abstract this experiment was carried out to determine the effects of proline and ascorbic acid on chilling injury, electrolyte leakage, lipid peroxidation, peroxide hydrogen and antioxidant enzymes activity of thompson grape (vitis vinifera l.) fruit. grape fruits were treated by 0 (control), 250 and 500 µm proline and 0 (control), 2 and 4% ascorbic acid and their combinations, then stored at 1...

this study evaluated the effect of the intermittent light program on serum and brain melatonin concentrations, antioxidant enzyme activities, and homocysteine concentration in broiler chickens. a total of 60 one-day-old broiler chickens (cobb 500) were distributed in three light-proof controlled rooms (20 chicks per room). all birds were reared in continuous light until 3 days of age. then, chi...