Crystalline surface layer proteins (S-layer proteins) have considerable potential for the crystalline arrays in biotechnology, biomimetics and nonlife applications, including areas such as microelectronics and molecular nanotechnology. The extensive application potential of surface layers in nanobiotechnology is according to the particular inherent attributes of the single molecular arrays cons...

It is well established that hydrophobic signal sequences direct proteins into or across the endoplasmic reticulum membrane in eukaryotes and cell membranes in prokaryotes. Although it is recognized that eukaryote proteins are efficiently secreted by bacterial systems, the export of bacterial proteins by eukaryotes has received little attention. To investigate membrane translocation of bacterial...

The sensitivity of growing bacterial cells to destructive agents or influences is many times that of bacterial spores (Curran, 1952). Some of these agents act on sulfhydryl groups of proteins; on the other hand, sulfhydryl groups belong to the most sensitive groups in proteins and are vital for the function of many enzymes (Barron, 1951). The question may be raised whether the degree of resista...

borreliosis is a bacterial zoonosis transmitted by arthropods. the infection is more common in dogs than other domestic animals. a one-month-old puppy with fever, jaundice and digestion disorder was referred to small animal hospital. laboratory results included severely regenerative anemia, neutrophilia, thrombocytopenia, strong spirochetemia and significant changes in serum proteins. the attem...

background and aims: among the bacterial agents, the most common cause of diarrheal disease is entrotoxigenic escherichia coli. letal toxin b (ltb) subunit of lt toxin could induce six-month immunity. clostridium botulinum causes botulism disease. bont/a-hc toxin subunit could induce two years immunity against this disease. it seems that the immunogenicity potency of these two subunits may infl...

Different chemical and physical methods are used for extraction of proteins from bacteria, which are used in variety of fields. But on a large scale, many methods have severe drawbacks. Recently, extraction by means of electroporation showed a great potential to quickly obtain proteins from bacteria. Since many parameters are affecting the yield of extracted proteins, our aim was to investigate...

The primary treatment modality for cancer involves surgical resection of the tumor(s) followed by radiation and chemotherapy. In some cases where the cancer, particularly pancreatic cancer, has advanced to a stage that precludes surgery, chemotherapy remains the standard of care. There are two types of drugs that are normally used in chemotherapy, both types usually guided by rational or struct...

Prokaryotic cells synthesize a set of small, usually basic proteins that bind DNA and are collectively called histonelike proteins because their biochemical properties resemble eukaryotic histones. Whereas the eukaryotic histones have a well defined function in packaging DNA through restraining left-handed toroidal DNA supercoils in nucleosomes, the functions of the prokaryotic histone-like pro...