objective(s): the purpose of this study was to investigate the role of chloride channel protein 2 (clc-2) in glutamate-induced apoptosis in the retinal ganglion cell line (rgc-5). materials and methods: rgc-5 cells were treated with 1 mm glutamate for 24 hr. the expression of clc-2, bax, and bcl-2 was detected by western blot analysis. cell survival and apoptosis were measured by 3-(4,5-dimethy...

Background: Mesenchymal stem cells (MSCs) are undifferentiated with the ability of multi-potency, pluripotency, and self-renewal. MSCs show great promise in cancer therapy due to their unique features. MSC secrete various cytokines multifunctional properties, although roles unclear. Methods: We have investigated influence secreted from on KG-1 as a cell model acute myeloid leukemia (AML). For t...

Yiqi Yangyin complex (YYC), the homology of medicine and food, is comprised Polygonati Rhizoma, Lycii Fructus, Codonopsis Pilosula, Rehmanniae Radix. Herein, YYC polysaccharide treatment effectively attenuated progression MGH in a mice model-induced with estrogen progestogen. significantly relived hormonal disorders by reducing levels receptor α (ERα) progesterone (PR), substantially elevated p...

Background and Objectives: Apoptosis plays a key role in pathogenesis of various cardiovascular diseases due to loss of terminally differentiated cardiac myocytes. Therefore, the aim of this study was to investigate effects of combined dietary restriction and aerobic training on indices of myocardial apoptosis in male rats. Materials and Methods: In this study, 32 three-month-old male rats wer...

PURPOSE We performed a clinical study on ING1b gene expression and p53 gene status in relation to p53 target genes. EXPERIMENTAL DESIGN Eighty-eight tumors from surgically treated non-small cell lung cancer (NSCLC) patients were studied. PCR-single-strand conformational polymorphism after sequencing was performed to investigate ING1 and p53 gene status. Quantitative reverse transcription-PCR ...

Objective(s): Liver transplantation is the most important therapy for end-stage liver disease and ischemia reperfusion (I/R) injury is indeed a risk factor for hepatic failure after grafting. The role of miRNAs in I/R is not completely understood. The aim of this study was to investigate the potential protective role of the mesenchymal stem cells (MSCs) and ischemic pr...

Objective(s): Several researchers have reported the relationship between infertility in male and varicocele for so many years but the implication of varicocele in female patients is remains elusive. Here, we aim to examine the effects of unilateral varicose ovarian vein on antioxidant capacity and oocyte quality of rat ovary after the experimental creation of varicocele in female rats. Material...

Background: It seems that Vinca. herbacea has an anti-tumor effect. Here, the immunotherapeutic effect of this compound is assessed against human ovarian cancer (SKOV3) cells because of the high incidence of this tumor in women. Materials and Methods: The cytotoxic activity of V. herbacea extract against human ovarian cancer (SKOV3) cells was determined by MTT assay. The apoptosis-inducing pote...

BACKGROUND Bax and Bcl-2 are the major members of Bcl-2 family whose play a key role in tumor progression or inhibition of intrinsic apoptotic pathway triggered by mitochondrial dysfunction. Therefore, the balance between pro- and anti-apoptotic members of this family can determine the cellular fate. METHODS In this study, the relative level of mRNA expression of Bax and Bcl-2 genes was deter...