Within the cereal aleurone reserve proteins are stored in specialized organelles, the protein storage vacuoles (PSV). We developed an aqueous method for the isolation of intact PSV. Barley (Hordeum vulgare L. cv Himalaya) aleurone protoplasts were gently lysed by passing them through a syringe needle. PSV were separated from cytoplasmic components by microfiltration and low-speed centrifugation...

Within the cereal aleurone reserve proteins are stored in specialized organelles, the protein storage vacuoles (PSV). W e developed an aqueous method for the isolation of intact PSV. Barley (Hordeum vulgare 1. cv Himalaya) aleurone protoplasts were gently lysed by passing them through a syringe needle. PSV were separated from cytoplasmic components by microfiltration and low-speed centrifugatio...

An inbreeding line of white clover has been identified which remains non-nodulated under appropriate physiological conditions and so the nitrogen concentration of the plant can be manipulated by altering the nitrate supply to the roots. Non-nodulating plants were used to test the hypothesis that acclimation to nitrogen limitation in white clover involves changes in protease activity and composi...

objective(s):  this study was undertaken to investigate the protective effects of vitamin b6, cofactor for cystathionine-γ lyase and cystathionine-β synthase (producers of h2s), alone and in combination with l-cysteine, h2s precursor, on indomethacin-, and ethanol-induced gastric lesions in male nmri mice. materials and methods: fasted male nmri mice were randomly assigned into 12 groups (7 in ...

Cystatin F is a cysteine peptidase inhibitor which, unlike other cystatin family members, is targeted to endosomal/lysosomal compartments. It is synthesized as an inactive disulfide-linked dimer which is then converted to an active monomer by proteolytic cleavage of 15 N-terminal residues. Cystatin F has been suggested to regulate the cytotoxicity of natural killer (NK) cells by inhibiting the ...

Osteoarthritis is the fourth leading cause of disability affecting more than 300 million people around globe. In this disease, chondrocytes responsible for growth and maintenance articular joints start to deplete due rogue involvement phosphoinositide 3-kinase, protein kinase-B p38 mitogenactivated kinases. Presently, there are medications providing symptomatic treatment, however, they cannot t...

Gamma-enolase enzymatic activity is involved in glycolysis, a prevalent process cancer cell metabolism. Additionally, gamma-enolase has pro-survival function, exhibited through the active site at C-terminal end of molecule. This regulated by cysteine peptidase cathepsin X, which cleaves two amino acids gamma-enolase. In clinical practice, determination as tumour marker does not differ between t...