The cyclin-dependent kinase inhibitor p21 is involved in a variety of different cellular processes, like cell cycle control, senescence and terminal differentiation. Recently, p21 was found to localize to discrete sites of DNA damage induced by heavy ions [1]. To investigate the functional significance of p21 at the DNA lesions, we designed a mammalian expression construct encoding translationa...

BACKGROUND AND AIM Rabies is a widespread neurological zoonotic disease causing significant mortality rates, especially in developing countries. Although a vaccine for rabies is available, its production and scheduling are costly in such countries. Advances in recombinant DNA technology have made it a good candidate for an affordable vaccine. Among the proteins of rabies virus, the Glycoprotein...

the use of l1 in language classroom has been traditionally repressed by applied linguists. however, in recent years, it is believed that switching to l1 can be considered a pedagogical instrument by teachers rather than a heterodox. consequently, this two-phase study was an attempt to investigate the functions that are fulfilled by efl teachers through their use of l1. in the first phase (i.e.,...

Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. Therefore, designing new and effective therapeutics is of great importance. The aim of the current study was to construct a DNA vaccine containing structural proteins of HCV and evaluation of its expression in a eukaryot...

Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...

Several authors demonstrated that an oligonucleotide based pH-sensitive construct can act as a switch between an open and a closed state by changing the pH. To validate this process, specially designed fluorescence dye-quencher substituted oligonucleotide constructs were developed to probe the switching between these two states. This paper reports on bulk and single molecule fluorescence invest...

Betel nut (BN), a natural carcinogen to humans, is used as a masticator across the globe by a large segment of the human population. The primary carcinogens of BN are alkaloids, in particular arecoline. Upon nitrosation, arecoline can potentially interact with DNA, forming adducts and initiating carcinogenesis. Though considerable evidence exists in support of the carcinogenicity of BN, the mol...

introduction: toxoplasma gondii is a protozoan parasite which is globally prevalent in human and animals. toxoplasma infection is commonly asymptomatic, but can cause serious medical problems in immunocompromised individuals and in fetus. recombinant antigens of the parasite may be helpful in diagnosing the infection more precisely. the goal of this study was to construct and evaluate the funct...