A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli s...

conclusions our study emphasized the increasing role of esbl genes, especially blactx-m15 in epec isolates. results of 42 epec, eight isolates carried the blactx-m1. none of the isolates carried blactx-m2 and blactx-m9. the blactx-m15 variant was identified in all of blactx-m1-positive isolates. furthermore, blashv and blatem genes were detected in 40.5% (n = 17) and 19% (n = 8) of all epec iso...

conclusions the activities studied by the production of pnp were determined by following the increase at 410 nm. the maximum pnp was produced at 30°c with an optical density of 10.62 in the presence of cytoplasmic expression of oph (pet21a-opd). consequently, our results suggest cytoplasmic expression system as an appropriate candidate with a high amount of oph in spite of inclusion body format...

conclusions this study indicated the contamination of lettuce by stec strains and its possible role as the source of infection. resistance to both tetracycline and ampicillin may be considered as an emergency alarm for a multidrug resistance of stec strains. results eight samples were positive for presence of stec strains, three contained stx1, five contained stx2, and one sample was positive f...

results in this study, high resistances were found to nalidixic acid (59.7%) and gentamicin (52.3%) but high susceptibilities were found to ciprofloxacin (59.3%) and ceftriaxone (36.3%). conclusions according to the results obtained in this study ciprofloxacin is an effective antibiotic in e. coli infections. objectives the current study aimed to investigate the resistance of escherichia coli a...

background infectious diarrhoeal diseases cause major problems throughout the world and are responsible for considerable morbidity and mortality. enteropathogenic escherichia coli (epec) may cause infantile diarrhoea among children in developing countries. objectives the aim of this study was to define the prevalence of epec strains in raw milk samples. materials and methods raw milk samples co...

background and objectives: pathogenic strains of escherichia coli are a common cause of acute infectious diarrhea. the aim of this study was to investigate the frequency, virulence markers and antibiotic resistance patterns of diarrheagenic e. coli (dec) isolated from adolescents and adults in hamadan, west of iran. materials and methods: a total of 187 stool samples were collected from adults ...

background: enterohemorrhagic escherichia coli (ehec) o157:h7 is an infectious zoonotic pathogen causing human infections. these infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. the first intimate bacterial adhesion, intimin (i), with its own receptor translocated intimin receptor (tir) and e. coli secreted protein...

Shigella species and Escherichia coli are closely related organisms. Early phenotyping experiments and several recent molecular studies put Shigella within the species E. coli. However, the whole-genome-based, alignment-free and parameter-free CVTree approach shows convincingly that four established Shigella species, Shigella boydii, Shigella sonnei, Shigella felxneri and Shigella dysenteriae, ...