Many real-world multilayer systems such as critical infrastructure are interdependent and embedded in space with links of a characteristic length. They are also vulnerable to localized attacks or failures, such as terrorist attacks or natural catastrophes, which affect all nodes within a given radius. Here we study the effects of localized attacks on spatial multiplex networks of two layers. We...

BACKGROUND This study describes the first multiplex real-time polymerase chain reaction assay developed, as a multipurpose assessment, for the simultaneous quantification of total bacteria and three Vibrio spp. (V. parahaemolyticus, V. vulnificus and V. anguillarum) in fish and seawater. The consumption of raw finfish as sushi or sashimi has been increasing the chance of Vibrio outbreaks in con...

Large-scale studies of biomolecular interactions required for proteome-level investigations can benefit from a new class of emerging surface plasmon resonance (SPR) sensors: nanohole arrays and surface plasmon (SP) enhanced optical transmission. In this paper we present a real-time, label-free multiplex SPR imaging sensor in a microarray format. The system presented is built around a low-cost m...

mutations in the dystrophin gene cause duchenne muscular dystrophy (dmd), the most commonly inherited neuromuscular disorder, and becker muscular dystrophy (bmd), the milder allelic form of the disease. the mutation spectrum within this gene is unusual in that deletion of one or more exons are found in ~65% of cases. since no effective treatment is so far available for these diseases, the ident...

Abstract. Sophian A, Purwaningsih R, Igirisa RPJ, Amirullah ML, Lukita BL, Fitri RA. 2020. Short Communication: Detection of Salmonella typhimurium ATCC 14028 and Listeria monocytogenes 7644 in processed meat products using Real-Time PCR Multiplex Method. Asian J Nad Prod Biochem 21: 17-20. The detection was carried out (qPCR) the Microbiology Molecular Biology Laboratory at Indonesian Food Dru...

developed in 1991, nucleic acid sequence-based amplification (nasba) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than pcr, and it can also be more sensitive. this paper describes the development of a molecular beacon-based multiplex nasba assay for simultaneous detection of hiv-1 and hcv in plasma samples.a well-conserved region...

A real-time PCR assay previously developed for use on the Roche LightCycler platform was investigated as an alternative to culture for the direct detection of vancomycin-resistant enterococci (VRE) in clinical specimens. PCR primers and fluorescence resonance energy transfer hybridization probes specific for the vanA and vanB genes were combined in a multiplex real-time PCR assay performed dire...

PURPOSE The purpose is to develop a real-time multiplex reverse transcription-PCR assay for detection and quantification of leukemia-specific chimeric transcripts that identify the genetic subgroups of acute lymphoblastic leukemias (ALLs) proposed by the WHO classification. EXPERIMENTAL DESIGN Real-time multiplex assay for t(12;21), t(4;11), and t(1;19) with hypoxanthine phosphoribosyltransfe...