Samples were tested for enterovirus by nucleic acid sequence-based amplification (NASBA) (NucliSens Basic kit; BioMerieux), reverse transcription-PCR (RT-PCR) (Enterovirus Consensus RT-PCR kit; Argene Biosoft), and virus isolation. Eighty-two samples were tested, and 44 were positive, 34 by both NASBA and RT-PCR and 5 each by NASBA or RT-PCR only. Two nasopharyngeal samples positive only by RT-...

the objective of the current study was to evaluate the reproducibility of a reverse transcriptase pcr (rt-pcr)-based technique to differentiate viable and dead salmonella cells in raw and sterilized milk. the microorganism was initially inoculated into the milk samples followed by incubating at 37°c for 4 h prior to inactivation by heat at 80°c for 10 min. the treated and non-treated samples we...

Bovine respiratory syncytial virus (BRSV) is a pneumovirus in the family paramyxoviridae, is an important cause of acute respiratory disease in postweaning calves and feedlot cattle. The real-time reverse transcriptase PCR protocols were developed to detect BRSV infection in infected animals. The sensitivity of RT-PCR protocols based on fusion gene were evaluated using different Mastermixes suc...

enteroviruses are the causative agents of a number of diseases in humans. group b coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. genomic data of enteroviruses has allowed developing new molecular approaches such as nucleic acid sequence based amplification (nasba) for detection of such viruses. in this study, coxsackievirus b3 (cvb3) was de...

abstract potato virus s is a member of the genus carlavirus in the family of flexiviridae, with curved filamentous particles 650×12 nm and positive single stranded rna genome. this virus is one of the most common viruses that infected all of potato varieties in the world. susceptible host species belong mainly to the families solanaceae, chenopodiaceae and amaranthaceae. potato virus s transmit...

Three methods to quantify gene transcript levels in mast cells, real-time RT-PCR, competitive RT-PCR and conventional RT-PCR analyses, were compared. Linear regression analysis on five gene transcripts revealed that the mRNA levels measured by real-time RT-PCR analysis were minimally correlated with those by conventional RT-PCR analysis. In addition, differences in the mRNA level between sample...

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

Background and Aims: The causal agents of viral lettuce big vein disease are two viruse, Lettuce big vein associated virus (Varicosavirus) and Mirafiori lettuce virus (Ophiovirus). These viruses have coat proteins of similar size but have different morphologies and serologically unrelated.The purpose of this study was to distinguish and detect LBVaV and MiLV in lettuce fields in Tehran Province...