The composition of a Brazilian green propolis ethanolic extract (Et-Bra) and its effect on Trypanosoma cruzi trypomastigotes and other pathogenic microorganisms have already been reported. Here, we further investigated Et-Bra targets in T. cruzi and its effect on experimental infection of mice. The IC(50)/4 days for inhibition of amastigote proliferation was 8.5 ± 1.8 μg mL(-1), with no damage ...

The aim of this study was to determine the effect of two variants of Tris-citric acid-fructose (TCF) extender containing whole hen egg yolk (TCF-HEY) and lyophilized lipoprotein fractions extracted from ostrich egg yolk (TCF-LPF(o)) on selected biological properties of cryopreserved sperm cells. Post-thaw percentage of motile sperm (MOT) was significantly higher (P < 0.05) for TCF-HEY extender ...

four mature stallions were used to study the effect of enzymatic antioxidant catalase on freezability of turkmen stallion&apos;s sperm. collected semen from stallions were processed and pooled before being divided treatments (200×106  sperm/ml) and diluted with extenders supplemented with different levels of catalase [0 (control), 100 u/ml (cat100), 150u/ml (cat150) and 200u/ml (cat200)]. exten...

Endoplasmic Reticulum (ER) is the largest and one of most complex cellular structures, indicating its widespread importance variety functions, including synthesis membrane secreted proteins, protein folding, calcium storage, lipid biogenesis. Moreover, ER implicated in cholesterol, plasmalogen, phospholipid, sphingomyelin biosynthesis. Furthermore, contact with organelles, such as mitochondria,...

Results of the studies carried out on localization and photodynamic action of merocyanine 540 (MC540) on carcinoma of cervix (HeLa) cells are presented. Fluorescence microscopic study showed that when HeLa cells were incubated with MC540 in dark, the dye localized in plasma membrane of cells. Photoirradiation of cells in presence of MC540 led to enhancement of dye uptake, intracellular localiza...

Since, there is only few documented evidence referring to assessment of the clear specific actions of medicinal plant source or active constituents in rodent model, the present study is undertaken to evaluate the effective concentration of technical azadirachtin-A, a tetranortritarpinoid of neem seed kernel, to immobilizes the cauda epididymal spermatozoa within 20 seconds and effectively inhib...

Antioxidants partially ameliorated the negative effects of reactive oxygen species (ROS) produced during cryopreservation. The objective of the present study was to investigate the effect of reduced glutathione, ascorbic acid and α-tocopherol on the quality of frozen-thawed buck spermatozoa. Ejaculates were collected by artificial vagina from 8 bucks and microscopically evaluated at 37 0 C. The...

Thioridazine (TZ) is used mainly in the treatment of schizophrenia. However, hepatotoxicity as a life-threatening adverse effect is associated with its clinical use. In this context, we examined the cytotoxic mechanisms of TZ on freshly isolated rat hepatocytes to better understanding of the pathogenesis of TZ-induced hepatotoxicity. Hepatocytes were prepared by the method of collagenase enzyme...

Plant cells possess a number of membrane bound organelles that play important roles in compartmentalizing a large number of biochemical pathways and physiological functions that have potentially harmful intermediates or by-products. The plasma membrane is particularly important as it holds the entire cellular structure whole and is at the interface between the cell and its environment. Conseque...

Basement membranes are specialized extracellular matrices consisting of tissue-specific organizations of multiple matrix molecules and serve as structural barriers as well as substrates for cellular interactions. The network of collagen IV is thought to define the scaffold integrating other components such as, laminins, nidogens or perlecan, into highly organized supramolecular architectures. T...