be established between recombinant and cellular protein biosynthesis, then stable and prolonged recombinant protein production is possible. Extending recombinant protein formation is the key to increased product yields. Five periods of doubling are needed to achieve maximum specific content. The specific content numbers can be derived from the kinetics of the recombinant protein production (4)....

background: parathyroid hormone is an 84-amino acid peptide secreted by the parathyroid glands. its physiological role is maintenance of normal serum calcium level and bone remodeling. biological activity of this hormone is related to n-terminal 1-34 amino acids. the recombinant form of hormone (1-34) has been approved for treatment of osteoporosis from 2002. in this study, a novel fusion partn...

chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. on the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into escherichia coli along with human basic fibroblast grow...

after using 3 different generations of antibodies including human and non-human hyperimmune sera, monoclonal antibodies and chimeric antibodies, more recently a newer approach has been developed in which the antibody genes are cloned directly from a patient peripheral b-lymphocytes and expressed in a host like e. coli. in this study the candida albicans serotype a (nctc 3153) mannan was purifie...

bioconversion of cellulosic material into bioethanol needs cellulase complex enzymesthat contain endoglucanase, exoglucanase and beta glucosidase. one of the most important organisms that produce cellulases is the filamentous fungi, trichoderma reesei which able to secrete large amounts of different cellulases. these enzymes are probably the most widely used cellulases industrially, however, th...

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

background: baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. the presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. methods: the aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

Background: Follicle-stimulating hormone (FSH) belongs to the family of glycoprotein hormones that composing alpha and beta subunits with non-covalently bonds. This hormone involve in regulation of the reproductive processes such as gamete generation and follicular growth. Injection of the hormone in most of fish species increases 17 beta-estradiol production by ovarian tissue and also stimulat...

Background: Conventional anti-cancer treatments, such as surgery, chemotherapy, radiation therapy, etc., are linked to antimicrobial resistance (AMR) and have negative effects on healthy tissues. Objectives: The flow cytometry technique was used in this study to evaluate the effectiveness of a modified anti-cancer peptide in treating the AGS gastric cancer cell line. Methods: This study used ...