By sampling perturbed state vectors from each ensemble forecast at additional time levels shifted by ±τ (where τ is a selected interval) the analysis time, time-expanded (TES) can not only sample timing errors (or phase errors) but also triple size for covariance construction without increasing size. In this study, TES was applied to convection-allowing ensemble-based warn-on-forecast system (W...

The E12 epoxy method of sheet plastination for preparing thin, transparent and serially sectioned cadaveric teaching specimens has been utilised primarily for studying sectional anatomy and correlation of MRI and CT radiographic images. The unique properties of the E12 process offer an exceptionally vivid survey of the human body in any given plane resulting in precise deliniation of the struct...

PURPOSE In this study, we used laser capture microdissection (LCM) and microarray hybridization technology to compare the gene expression profiles of mouse embryonic days 10 and 12 lenses (E10 and E12). METHODS Lens cells of C57/BL6 mouse embryos at E10 and E12 were harvested using the PixCell II LCM System. Total RNA was extracted, amplified, labeled, and hybridized to the 430 2.0 mouse chip...

The development of synaptic function was examined at auditory nerve synapses in the rostromedial region of the cochlear nucleus magnocellularis of the chick. EPSCs were studied beginning at embryonic day 12 (E12), when synaptic transmission was first observed, through E19. The amplitude of evoked EPSCs produced by AMPA receptor (AMPA-R) increased 30-fold over this age range, whereas NMDA recept...

A T-lymphocyte-specific enhancer located 13 kb upstream of the murine CD4 gene was recently shown to be required for the developmentally regulated expression of CD4. We have previously identified three nuclear protein binding sites in this enhancer; one of these sites, CD4-3, is essential for expression and contains two E-box core motifs (CANNTG) adjacent to each other in the sequence TAACAGGTG...

It is commonly held that hearing generally begins on incubation day 12 (E12) in the chicken embryo (Gallus domesticus). However, little is known about the response properties of cochlear ganglion neurons for ages younger than E18. We studied ganglion neurons innervating the basilar papilla of embryos (E12-E18) and hatchlings (P13-P15). We asked first, when do primary afferent neurons begin to e...