OBJECTIVE Annexin A5 is a phosphatidylserine binding protein that binds dying cells in vivo. Annexin A5 is a potential molecular imaging agent to determine efficacy of anti-cancer therapy in patients. Its rapid clearance from circulation limits tumor uptake and, hence, its sensitivity. The aim of this study is to determine if non-invasive imaging of cell death in tumors will benefit from increa...

1 0 2 0 1 1 (a) Suppose a2 = (1, 2, 2) , a3 = (−2, 0, 1) , a4 = (0, 4, 5) , a5 = (0, 1, 1) . Compute the ranks of A and B. Explain why B can not be obtained from A by a finite number of elementary row operations. Solution: The first 3 columns of B are independent, so its column space has dimension 3, thus rank(B)=3. The second, third and fifth column of A are independent, so its column space ha...

For example, on input a1, a2, a3, a4, a5 where a1 = 3, a2 = 4, a3 = 4, a4 = 5, a5 = 7, a possible solution is to take S1 = {1, 4} and S2 = {2, 3}, bacause a1 + a4 = a2 + a3 = 8. On the other hand, on input the sequence 2, 3, 4, there is no possible solution. Devise a dynamic programming strategy to decide whether such sets S1 and S2 exists. The solution should run in time polynomial in n and M ...

(99m)Tc-HYNIC-annexin A5 can be considered as a benchmark in the field of apoptosis imaging. However, (99m)Tc-HYNIC-annexin A5 has characteristics of high uptake and long retention in non-target tissues such as kidney and liver. To minimize this problem, we developed a novel (99m)Tc-labeled annexin A5 using a bis(hydroxamamide) derivative [C3(BHam)2] as a bifunctional chelating agent, and evalu...

A novel annexin A5 derivative (cys-annexin A5) with a single cysteine residue at its C-terminal has been developed and successfully labeled in high labeling yield with (99m)Tc by a ligand exchange reaction. Like the 1st generation (99m)Tc-HYNIC-annexin A5, the novel (99m)Tc-cys-annexin A5 derivative shows in normal mice mainly renal and, to a lesser extent, hepatobiliary excretion. In rat model...

Proteins of the annexin family bind to phospholipids in a Ca(2+) dependent manner. The exposure of phosphatidylserine (PS) by apoptotic as well as necrotic cells is one major eat-me-signal for macrophages. Annexin A5 (Anx A5) preferentially binds to PS. The availability of Anx A5 knock out (KO) mice allowed us to investigate for the first time if endogenous Anx A5 modulates the immune response ...

the biocontrol agents candida  membranifuciens (isolates a4 and a5), pichia  guilliermondi (a6) and bacillus subtilis (b2 and b6) were tested both individually and in combination for biological control of the grey mold in fruit apple caused by botrytis cinerea pers.:fr. regarding the compatibility of the biocontrol agents, it showed that antagonists were compatible with each other according to ...