A new species of Acanthamoeba was isolated from a freshwater fish in Korea and tentatively named Acanthamoeba sp. YM-4 (Korean isolate YM-4). The trophozoites were 11.0-23.0 micrometer in length and had hyaline filamentous projections. Cysts were similar to those of A. culbertsoni and A. royreba, which were previously designated as Acanthamoeba group III. Acanthamoeba YM-4 can survive at 40 deg...

Forty eyewash units were sampled for protozoa, bacteria, and fungi. Total heterotrophic bacterial counts on nutrient agar and R2A agar (Difco Laboratories, Detroit, Mich.) ranged from 0 to 10(5) CFU/ml, with Pseudomonas spp. being the most frequently isolated. Total counts of 10(4) and 10(8) cells per ml were obtained with the acridine orange staining procedure. All samples were examined for Le...

PURPOSE Developing a DNA dot hybridization model for diagnosing parasitic keratitis. METHODS Newly designed oligonucleotide probes for detecting Acanthamoeba and microsporidia were tested with target reference strains of Acanthamoeba (n = 20) and microsporidia (n = 3), and non-target microorganisms, including bacteria (n = 20) and fungi (n = 20). These probes, which had passed the preliminary...

acanthamoeba keratitis is a vision-threatening infection caused by pathogenic species of the genus acanthamoeba. in this study, 13 acanthamoeba keratitis cases were diagnosed among 52 keratitis patients. to confirm the identity of acanthamoeba at the genus level, a pcr-based method was used, and their pathogenic potential was determined using in vitro cytotoxicity assays on human corneal epithe...

Abstract The current study presents a comprehensive review of worldwide waterborne parasitic protozoan outbreaks reported between 2017 and 2022. Totally, 416 were attributed to transmission protozoa. Cryptosporidium was the etiological agent in 77.4% (322) outbreaks, while Giardia 17.1% (71). Toxoplasma gondii Naegleria fowleri causal 1.4% (6), 1% (4) respectively. Each Blastocystis hominis, Cy...

Introduction Definition Free-living amebae of the genera Naegleria, Acanthamoeba, and Balamuthia cause fatal diseases of the central nervous system (CNS) of humans.1-33 Naegleria fowleri causes an acute and fulminant primary amebic meningoencephalitis (PAM) in children and young adults with a history of exposure to fresh water leading to death within 5 to 10 days after the onset of symptoms.4-6...

A survey was carried out from August to December 2004 in Pusan, Korea to document the presence of free-living amoeba (FLA), including the genus Acanthamoeba, in both contact lens storage cases and domestic tap water. Acanthamoeba was isolated from 5 (4.2%) in 120 contact lens storage cases. Four house tap water samples from residents, whose contact lens storage cases had been contaminated by Ac...