background: trichomoniasis, caused by trichomonas vaginalis , is one of the most common sexually transmitted infections in the world. diagnosis of t. vaginalis is performed by different methods, including wet mount, culture, serological methods and pcr, which required laboratory equipments and expert laboratory personnel. the aim of this study was evaluation of immunochromatographic strip test ...

In efforts to prepare more potent and sensitive viral serological antigens, several aspects of the production of antigens from infected cell cultures were studied. Antigens derived from whole, infected culture material and from the cellular and fluid phases were compared. Freezing and thawing, sonication, and alkaline buffer extraction were compared for effectiveness in releasing antigen from h...

conclusions the results prove that the presented double pcr method could be used to detect positive cases from culture-negative blood samples. the chelex 100 method is simpler and safer than the use of qiagen kit for dna extraction. materials and methods a total of 100 patients with suspected brucellosis were included in this experimental study and given positive serological tests. diagnosis wa...

The serological diagnosis of melioidosis is carried out using the indirect hemagglutination assay. We looked at the reactivity of sera from culture-proven cases of melioidosis from north Queensland against antigens derived from Burkholderia pseudomallei, B. thailandensis, and B. cepacia. Cross-reactivity between sera from culture-positive cases of melioidosis and B. thailandensis was demonstrated.

BACKGROUND During pulmonary tuberculosis (PTB) antibodies are generated to trehalose esters of mycolic acids which are cell wall lipids of Mycobacterium tuberculosis (Mtb). Attempts have been made to use these complex natural mixtures in serological tests for PTB diagnosis. AIM The aim of this work was to determine whether a serological test based on a panel of defined individual trehalose es...

visceral leishmaniasis (vl) is a zoonotic disease caused by leishmania species. dogs are considered to be the main reservoir of vl. a number of methods and antigen-based assays are used for the diagnosis of leishmaniasis. however, currently available methods are mainly based on direct examination of tissues for the presence of parasites, which is highly invasive. a variety of serological tests ...

This study aimed to investigate the prevalence of human brucellosis in Najran, southwestern Saudi Arabia, and to assess the performances of ELISA and PCR as diagnostic tools for brucellosis with respect to conventional methods. The study included 340 patients with clinical characteristics of brucellosis. Blood samples from cases and controls were subjected to culture, standard tube agglutinatio...