ABSTRACT Ninety-five isolates of Colletotrichum including 81 isolates of C. acutatum (62 from strawberry) and 14 isolates of C. gloeosporioides (13 from strawberry) were characterized by various molecular methods and pathogenicity tests. Results based on random amplified polymorphic DNA (RAPD) polymorphism and internal transcribed spacer (ITS) 2 sequence data provided clear genetic evidence of ...

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular and morphological aspects of Parabronema skrjabini in sheep and goats in Iran. The worms were collected from these animal species from three different regions. An internal transcribed spacer 2 riboso...

in this study, the full-length m2 gene of the avian influenza virus (h9n2) was isolated, analyzed and studied in detail. total rna was extracted and cdna of the m2 mrna was obtained by reverse transcriptase polymerase chain reaction (rt-pcr) using random hexamer oligoes; specific primers were used for amplification of the m2 open reading frame (orf) region. pcr was able to amplify the desirable...

ABSTRACT The stem nematode Ditylenchus dipsaci is of great economic importance worldwide as a parasite of agricultural crops and horticultural plants. The internal transcribed spacer (ITS) of rDNA from 23 populations of the D. dipsaci complex from various host plants were amplified and sequenced. Seven previously studied populations were also included in the study. The phylogenetic analysis of ...

An intraspecific study of Phlebotomus sergenti was performed on populations from Turkey, Syria, Israel, and Uzbekistan by four different approaches: geometric morphometrics, RAPD analysis, internal transcribed spacer 2 (ITS2) sequencing (nuclear marker), and cytochrome B sequencing (mitochondrial marker). In RAPD analysis, distinct clades were formed in accordance with the geographical origin o...

Trichoderma spp. are fungus considered one of the most attractive for biological control due to its different mechanisms action against plant pathogens and promote growth productivity. The molecular characterization this group antagonists is great importance conducting studies in area biodiversity identifying selecting possibility their use control. Objective work was identify 29 isolates based...

Abstract Protothecosis is an infectious disease caused by organisms currently classified within the green algal genus Prototheca . The can manifest as cutaneous lesions, olecranon bursitis or disseminated systemic infections in both immunocompetent and immunosuppressed patients. Concerning diagnostics, taxonomic validity important. , closely related to Chlorella species complex, known be polyph...

objective(s):brucellosis is a well-known domestic animal infectious disease, which is caused by brucella bacterium. groel antigen increases brucella survival and is one of the major antigens that stimulates the immune system. hence, the objective of the present study was cloning and bioinformatics analysis of groel gene. materials and methods: the full-length open reading frame of this gene was...

BACKGROUND Herbal drugs used to treat illness according to Ayurveda are often misidentified or adulterated with similar plant materials. OBJECTIVE To aid taxonomical identification, we used DNA barcoding to evaluate authentic and substitute samples of herb and phylogenetic relationship of four medicinal plants of family Asparagaceace and Asclepiadaceae. MATERIALS AND METHODS DNA extracted f...