نتایج جستجو برای: mlva technique

تعداد نتایج: 611850  

2015
Adrian Allen Eleanor Breadon Andrew Byrne Thomas Mallon Robin Skuce Pauline Groussaud Amanda Dainty Judith Graham Kerri Jones Lorraine Pollock Adrian Whatmore Axel Cloeckaert

BACKGROUND Brucellosis is the most common bacterial zoonoses worldwide. Bovine brucellosis caused by Brucella abortus has far reaching animal health and economic impacts at both the local and national levels. Alongside traditional veterinary epidemiology, the use of molecular typing has recently been applied to inform on bacterial population structure and identify epidemiologically-linked cases...

2014
Kinga M Sulyok Zsuzsa Kreizinger Lilla Fekete Szilárd Jánosi Nóra Schweitzer Ibolya Turcsányi László Makrai Károly Erdélyi Miklós Gyuranecz

BACKGROUND Mycoplasma bovis is an important pathogen causing pneumonia, mastitis and arthritis in cattle worldwide. As this agent is primarily transmitted by direct contact and spread through animal movements, efficient genotyping systems are essential for the monitoring of the disease and for epidemiological investigations. The aim of this study was to compare and evaluate the multi locus sequ...

2015
Sandra Moore Berthe Miwanda Adodo Yao Sadji Hélène Thefenne Fakhri Jeddi Stanislas Rebaudet Hilde de Boeck Bawimodom Bidjada Jean-Jacques Depina Didier Bompangue Aaron Aruna Abedi Lamine Koivogui Sakoba Keita Eric Garnotel Pierre-Denis Plisnier Raymond Ruimy Nicholas Thomson Jean-Jacques Muyembe Renaud Piarroux Ruifu Yang

BACKGROUND Since cholera appeared in Africa during the 1970s, cases have been reported on the continent every year. In Sub-Saharan Africa, cholera outbreaks primarily cluster at certain hotspots including the African Great Lakes Region and West Africa. METHODOLOGY/PRINCIPAL FINDINGS In this study, we applied MLVA (Multi-Locus Variable Number Tandem Repeat Analysis) typing of 337 Vibrio choler...

2015
Pei Xiao Hongxia Yang Dongdong Di Dongri Piao Qiuxiang Zhang Ruie Hao Suxia Yao Rong Zhao Fanfei Zhang Guozhong Tian Hongyan Zhao Weixing Fan Buyun Cui Hai Jiang

BACKGROUND Brucellosis presents a significant economic burden for China because it causes reproductive failure in host species and chronic health problems in humans. These problems can involve multiple organs. Brucellosis is highly endemic in Shanxi Province China. Molecular typing would be very useful to epidemiological surveillance. The purpose of this study was to assess the diversity of Bru...

Journal: :Japanese journal of infectious diseases 2008
Yingxin Pei Jun Terajima Yasunori Saito Reiko Suzuki Nobuko Takai Hidemasa Izumiya Tomoko Morita-Ishihara Makoto Ohnishi Masashi Miura Sunao Iyoda Jiro Mitobe Binyou Wang Haruo Watanabe

We identified seven distinct subtypes of enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were derived from sporadic cases and outbreaks from multiple prefectures in Japan in 2005. A surveillance system utilizing pulsed-field gel electrophoresis (PFGE), PulseNet Japan, was used. Some strains showed indistinguishable PFGE patterns using another restriction enzyme (BlnI or SpeI) in...

2014
Kinga M Sulyok Zsuzsa Kreizinger Heidie M Hornstra Talima Pearson Alexandra Szigeti Ádám Dán Eszter Balla Paul S Keim Miklós Gyuranecz

BACKGROUND Information about the genotypic characteristic of Coxiella burnetii from Hungary is lacking. The aim of this study is to describe the genetic diversity of C. burnetii in Hungary and compare genotypes with those found elsewhere. A total of 12 samples: (cattle, n = 6, sheep, n = 5 and human, n = 1) collected from across Hungary were studied by a 10-loci multispacer sequence typing (MST...

2013
Hai Jiang Heng Wang Liqing Xu Guiying Hu Junying Ma Pei Xiao Weixing Fan Dongdong Di Guozhong Tian Mengguang Fan Jingchuan Mi Ruiping Yu Litao Song Hongyan Zhao Dongri Piao Buyun Cui

In China, brucellosis is an endemic disease and the main sources of brucellosis in animals and humans are infected sheep, cattle and swine. Brucella melitensis (biovars 1 and 3) is the predominant species, associated with sporadic cases and outbreak in humans. Isolates of B. abortus, primarily biovars 1 and 3, and B. suis biovars 1 and 3 are also associated with sporadic human brucellosis. In t...

Journal: :Applied and environmental microbiology 2015
L Poulin P Grygiel M Magne L Gagnevin L M Rodriguez-R N Forero Serna S Zhao M El Rafii S Dao C Tekete I Wonni O Koita O Pruvost V Verdier C Vernière R Koebnik

Multilocus variable-number tandem-repeat analysis (MLVA) is efficient for routine typing and for investigating the genetic structures of natural microbial populations. Two distinct pathovars of Xanthomonas oryzae can cause significant crop losses in tropical and temperate rice-growing countries. Bacterial leaf streak is caused by X. oryzae pv. oryzicola, and bacterial leaf blight is caused by X...

Journal: :Genetics and molecular research : GMR 2016
R T Chideroli U P Pereira D D Gonçalves A Y Nakamura A A Alfieri A F Alfieri J C Freitas

Most epidemiologic studies on bovine leptospirosis are based on serological tests that use antibodies against several serotypes, including the serovar Hardjo, which is widespread and considered to be the most adapted to bovine hosts. However, using only serological studies is not sufficient to identify and distinguish species of leptospires. The aim of this study was report the first isolation ...

سابقه و هدف: سودوموناس آئروژینوزا یکی از شایع ترین عوامل عفونت­های ادراری در بیمارستان­ها می باشد. هدف از این مطالعه تعیین تیپ تعدادی از جدایه های بدست آمده از بیماران به روش MLVA به منظور رسیدن به یک الگوی ژنتیکی مشخص برای دسته بندی آنها می­باشد. مواد و روش ­ها: در این مطالعه 70 سویه سودموناس آئروژینوزا از نمونه ادراری بیماران در بیمارستان های تهران جمع آوری شد. برای بررسی ژنوتیپی بر اساس روش ...

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