نتایج جستجو برای: pcr reaction is almost twice compared with rt

تعداد نتایج: 11403121  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده زیست شناسی 1388

126 abstract: in this research we studied the effects of cyclic hydration and dehydration of cysts of artemia urmiana and artemia parthenogenetica on the hatching percentage, survival and growth. the experiment was carried out in 3 treatments (1-3 hydration/dehydration cycles) with 3 replicates for each treatment. later effects of cold preservation at -20°c during three time periods were ...

باغبان اسلامی نژاد , محمدرضا, تقی یار , لیلا, پیریایی , عباس,

Background and purpose: Vitro cartilage differentiation of mesenchymal stem cells (MSCs) has been noticed in several investigations. In this regard, almost always molecular differentiation of the cells has been examined, while structural and morphological differentiation of them has been ignored. Therefore, the present study examines the structure and ultrastructure of the cartilage different...

Introduction: N-nitroso-N-methylurea (NMU) is a carcinogen from nitrosamines family, which has been used to induce breast cancer in rodents. This model of breast cancer is very similar to the estrogen dependent breast cancer in human. As a continuation of our recent works, in the present study, the expressions of both p53 and p27 were investigated in NMU-induced breast cancer in Wistar Albin...

Journal: :Egyptian Journal of Medical Human Genetics 2021

Abstract Background The molecular mechanisms for chronic kidney disease (CKD) remain largely unknown and appear to be multifactorial. In the current study, we aimed study circulatory levels of circular ankyrin repeat domain 36 (circANKRD36) ANKRD36 in Egyptian patients with type 2 diabetes mellitus (T2DM) CKD explore their associations progression CKD. This cross-sectional controlled enrolled 6...

Journal: :Clinical chemistry 2014
Rana K Daher Gale Stewart Maurice Boissinot Michel G Bergeron

BACKGROUND Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplici...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه گیلان 1390

in iran we already use laminate tubes only in cosmetics industry. laminate tube manufacturers via using the most modern technology of the world, faced with the laminate tube market being saturated in cosmetics industry for packaging the cosmetic creams and toothpaste. but considering the great food market and therefore laminate tube features such as beautiful packaging, ease of use for the fina...

2011
F Abbasian H Tabatabaie M Sarijloo S Shahmahmoodi A Yousefi T Saberbaghi T Mokhtari Azad R Nategh

BACKGROUND AND OBJECTIVES Each year, Enteroviruses infect millions of people and cause different diseases. The agents are usually detected using cell culture. RD (Rhabdomyosarcoma) and L20B (L cells) are among the recommended cells by the World Health Organisation (WHO) for this purpose. Even though cell culture is the most common method used in diagnosing Enteroviruses in stool specimens, this...

Journal: :Molecular and cellular probes 2010
Hao-tai Chen Jie Zhang Yan-ping Ma Li-Na Ma Yao-zhong Ding Xiang-tao Liu Xue-peng Cai Li-qing Ma Yong-guang Zhang Yong-sheng Liu

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-reaction of IBV RT-LAMP was found when tested with other viruses including Newcas...

Journal: :iranian biomedical journal 0
اسماعیل صابرفر esmaeil saberfar محمد ام فرقانی فرد mohammad m. forghani-fard میرلطیف موسوی mirlatif mosavi

avian influenza virus (aiv) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. methods: a multiplex reverse transcriptase pcr (rt-pcr) was optimized for the detection of influenza a virus and the h5 and h9 subtypes. the influenza type a specific primers were directed to th...

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