Plasmid-to-chromosome ratios in Enterobacteriaceae, upon interruption of protein synthesis by chloramphenicol, are either conserved or increased when measured by dye buoyant density centrifugation. We have found, on the other hand, that the effect of inhibition of protein synthesis on the amount of covalently closed circular deoxyribonucleic acid visualized by this method in two strains of Stre...

RSF1010 plasmid DNA was introduced into Pseudomonas putida and P. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in Pseudomonas species. The number of copies of RSF1010 was 43 per chromosome equivalent in P. putida cells.

We describe a novel mechanism of site-specific recombination in the unicellular marine cyanobacterium Synechococcus sp. PCC7002. The specific recombination sites on the smallest plasmid pAQ1 were localized by studying the properties of pAQ1-derived shuttle-vectors. We found that a palindromic element, the core sequence of which is G(G/A)CGATCGCC, functions as a resolution site for site-specific...

Background & objective:  Tuberculosis (TB) remains a major cause of death around the world. Bacillus Calmette Guérin (BCG) is the only vaccine used in TB prevention that has a protective effect in children, but its effectiveness declines in adults. Design and development of new vaccines is the most effective way against TB. The aim of this study was to design and construc...

Antibiotic resistant and biofilm forming uropathogenic Enterobacteriaceae are rising. This study was conducted to evaluate the efflux pump plasmid mediated genes in ciprofloxacin (CIP) hospital acquired Escherichia coli Klebsiella pneumoniae. Also, assess anti-biofilm action of carbonyl cyanide m-chlorophenylhydrazone (CCCP). Uropathogenic E. K. pneumoniae isolates were collected from Mansoura ...

Bacterial conjugative plasmids could carry genes involved in pesticide biodegradation. However, its conjugal transfer is affected by stressors such as metals. Our aim was to determine if bacterial cells exposed copper can conjugation the catabolic plasmid pRO101 and assessed expression of traK gene. Cupriavidus pinatubonensis AEO106 Pseudomonas putida KT2440 were used donor recipient strains, r...

This study was aimed to determine the prevalence of ESBLs in E. coli and their relation plasmid profiling patterns a sample patients Iraqi hospitals. The total number isolates 91 (49%) were collected out 185 clinical samples. antibiotic sensitivity test for showed high resistance percentage Nitrofurantion 95.7%, amoxicillin Ceftriaxone each which 91.3% Ceftazidime 90.2%, while highest observed ...

The stable inheritance of bacterial plasmids is achieved by a number of different mechanisms. Among them are resolution of plasmid oligomers into monomers, active plasmid partitioning into dividing cells and selective killing of plasmid-free segregants. A special focus is given to the last mechanism. It involves a stable toxin and an unstable antidote. The antidotes neutralize their cognate tox...

plasmid DNA. Those fragments represent the homologous Ti-plasmid DNA segment 8,um long (1 5 % of the Ti-plasmid). An argument that this region of the plasmid contains genes essential for the expression of oncogenicity came from the analysis of a deletion mutant in the Ti-plasmid (Hernalsteens et al., 1975). Since this deletion mutant is unable to confer oncogenicity and has lost exactly those t...