Contemporary analytical methods have the sensitivity required for Ochratoxin A detection and quantification, but direct application of these methods on real samples can be rarely performed because of matrix complexity. Thus, efficient sample pre-treatment methods are needed. Recent years have seen the increasing use of artificial recognition systems as a viable alternative to natural receptors,...

Analytical Solutions and type curves for the constant rate radial flow of fluid in both conventional and naturally fractured reservoirs including the effect of wellbore phase redistribution are presented. An automated procedure for non-linear least square minimization using the analytical solutions and their derivatives with respect to the unknown parameters developed to analyze the pressur...

Phlebotomy is often addressed as a crucial process in the pre-analytical phase, in which a large part of laboratory errors take place, but to date there is not yet a consolidated methodological paradigm. Seeking literature, we found 36 suitable investigations issued between 1996 and 2016 (April) dealing with the investigation of pre-analytical factors related to phlebotomy. We found that the la...

The suitability of analytical protocol for determination of sulfonamides in honey was investigated. Sulfonamides were extracted from honey with acetate buffer, and cleaned up by solid phase extraction (SPE) procedure. The liquid chromatography (LC) separation was carried out on RP C18 column and sulfonamides were monitored with fluorescence detector, after pre-column derivatisation. Recoveries ...

Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV i...

A simple and effective analytical procedure was developed for the determination of fenthion residues in human serum samples. The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the advantage to require low amount of serum (1 mL) without tedious pre-treatment. The quantification of fenthion was carried out by gas chromatography-m...

Directive 98/79/EC guides laboratory medicine in Europe to large and preferable using properly tested and documented in vitro diagnostic medical devices and their accessories. From that point of view the role of bio-analysts working in clinical laboratories more closely associates with completely validated technologies, intra-laboratory verification of commercial methods and products, long-term...

The diagnostic use of in vitro molecular assays can be limited by the lack of guidelines for collection, handling, stabilization and storage of patient specimens. One of the major goals of the EC funded project SPIDIA (www.spidia.eu) is to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for molecular testing which requires intracellular RNA analytes....