1. Evidence has been found that Northrop's phenomenon (so called by us) is produced in the digestion of casein or hemoglobin brought about by trypsin, papain, and pepsin either crude or crystalline in the presence of gelatin. 2. Anson's and Kunitz' methods permit the measure of proteolytic activity of any protease on casein or hemoglobin substrate in the presence of gelatin, even in very small ...

(1'S*,2'S*)-(+/-)-6-Nor-2',3'-dihydro-4'-deoxo-ABA (2) was designed and synthesized as a candidate lead compound for developing a potent and specific inhibitor of ABA 8'-hydroxylase. This compound acted as an effective competitive inhibitor of the enzyme, with a K(I) value of 0.40microM, without exhibiting ABA activity. However, compound 2 also functioned as an enzyme substrate, making it a sho...

The proposed work aims to develop a sensitive surface-enhanced Raman spectroscopy (SERS) nano-biosensor. inverted nano pyramid array on silicon substrate fabricated using electron beam lithography (EBL) was utilised as master template and the mold later replicated via nanoimprinting process prepare gold-coated polymer nanopyramid three-dimensional (3D) SERS substrate. fast versatile replication...

The aim of the study was the demonstration and choice of conditions for the determination of cathepsin D activity in human mixed saliva. The 6% solution of hemoglobin, denatured with hydrochloric acid, was used as the substrate. The ratio of saliva volume to hemoglobin was 4:1 w/v. The reaction was interrupted by adding 10% trichloroacetic acid, after 6 hours of incubation at 37 degrees C. The ...

A new substrate, ammonium thymolphthalein monophosphate, is proposed for use in alkaline and acid phosphatase determinations. The ammonium salt shares the advantages of the sodium salt, but is a more stable substrate and results in more stable working reagents. The substrate is buffered at ph 10.1 in diethanolamine for determining alkaline phosphatase activity in 5 mm, and at pH 5.9 in citrate ...

background: β-thalassemia considers worldwide public health disorders. novel fetal hemoglobin inducer agents such as thalidomide and sodium butyrate have been attended for ameliorating clinical complications of such disorders. material and methods: we used thalidomide and sodium butyrate for increasing the level of fetal hemoglobin in erythroid progenitors. briefly, after isolation of cd133+ st...